This entry represents the RNA recognition motif (RRM) of TRMT2A (tRNA (uracil-5-)-methyltransferase homologue A), which is a cell cycle regulated protein that has been associated with the clinical outcome in HER2 expressing breast cancer []. The function of TRMT2A remains unclear although by sequence homology it has a RNA recognition motif (RRM).
This entry represents a family of tRNA (uracil-5-)-methyltransferase homologues (), mainly found in metazoa, including tRNA (uracil-5-)-methyltransferase homologue A (TRMT2A) and B (TRMT2B) from human. TRMT2A catalyses the formation of 5-methyl-uridine in tRNAs and some mRNAs [, , ]. TRMT2A/B mainly catalyse the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs [, ]. This family also includes the plant homologue AtC3H24 (also known as TRNA METHYLTRANSFERASE 2B) []and putative RNA methyltransferases R405/407 from Acanthamoeba polyphaga mimivirus.Methyltransferases (EC [intenz:2.1.1.-]) constitute an important class of enzymes present in every life form. They transfer a methyl group most frequently from S-adenosyl L-methionine (SAM or AdoMet) to a nucleophilic acceptor such as oxygen leading to S-adenosyl-L-homocysteine (AdoHcy) and a methylated molecule [, , ]. All these enzymes have in common a conserved region of about 130 amino acid residues that allow them to bind SAM []. The substrates that are methylated by these enzymes cover virtually every kind of biomolecules ranging from small molecules, to lipids, proteins and nucleic acids [, , ]. Methyltransferase are therefore involved in many essential cellular processes including biosynthesis, signal transduction, protein repair, chromatin regulation and gene silencing [, , ]. More than 230 families of methyltransferases have been described so far, of which more than 220 use SAM as the methyl donor.
