This entry represents a domain found in the Trm5/Tyw2-type methyltransferase. Trm5 and Tyw2 are S-adenosylmethionine-dependent methyltransferases involved in tRNA methylation []. Wybutosine synthesis is a multienzymatic process that comprises six sequential enzymatic reactions involving five enzymes. The initial step, the formation of m1G-37 (guanosine-37 methylated in the N1 position) in precursor tRNAPhe is catalysed by tRNA wybutosine-synthesising protein 5 (Trm5). Tyw2 catalyses the second step, consisting of the alpha-amino-alpha-carboxypropyl (acp) group being transferred from S-AdoMet to the side chain at the C7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 [].
This entry represents tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase TYW2 from archaea. It is a s-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. It catalyses the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe) [].
