tRNA wybutosine-synthesizing protein 5 (TYW5) is a tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. TYW5 catalyses the oxidation of wybutosine (yW), a hypermodified nucleoside found in position 37 of tRNA(Phe), to produce hydroxywybutosine (OHyW), a derivative found in eukaryotes []. It contains a JmjC domain, a domain that can be found in a large superfamily of Fe(II)/2-oxoglutarate (2-OG) dependent oxygenases. The structure of TYW5 has been solved [].
