Vacuolar protein sorting-associated protein 54 (Vps54) is a component of the GARP (Vps52/53/54) complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN) []. Mammalian GARP complex has been shown to play a general role in the delivery of retrograde cargo into the TGN []. In mice, a point mutation (L967Q) in the Vps54 gene causes wobbler mutation, which is characterised by degeneration of upper and lower motor neurons leading to progressive muscle weakness. The wobbler mouse is an animal model for human motor neuron disease, such as amyotrophic lateral sclerosis (ALS) []. Interestingly, the protein mutation (L967Q) does not prevent its integration into the GARP complex but greatly reduces the half-life and levels of the protein in vivo [].
This entry represents a domain found in vacuolar protein sorting-associated protein 54 (VPS54), which acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). VPS54 is required to tether the complex to the TGN. However, it is not involved in endocytic recycling [].
Vps52 complexes with Vps53 and Vps54 to form the Golgi-associated retrograde protein (GARP) complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network, regulating membrane trafficking events [, , ]. It is also part of the EARP (Endosome-Associated Recycling Protein) complex, involved in endocytic recycling [].
Vps53 complexes with Vps52 and Vps54 to form a multi-subunit complex called Golgi-Associated Retrograde Protein complex (GARP) involved in retrograde transport from early and late endosomes to late Golgi [, , ]. They are members of the CATCHR (complexes associated with tethering containing helical rods) which includes the exocyst, COG, GARP, and DSL1 complexes which have structural and functional similarities. This is the N-terminal coiled-coil domain [].
Syndetin (VPS50) is a component of the endosome-associated recycling protein (EARP) complex. This complex and the Golgi-associated retrograde protein (GARP) complex share four of the five subunits, but EARP contains syndetin in place of the Vps54 subunit of GARP, with the effect that the EARP complex is directed to endosomes rather than the Golgi. EARP in required for membrane-fusion during endocytic recycling of receptors and is described as a tethering complex [].
This entry represents a domain found in vacuolar protein sorting-associated protein 54 (VPS54), which acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). VPS54 is required to tether the complex to the TGN. However, it is not involved in endocytic recycling [].Proteins containing this domain also includes VPS50 (also known as Syndetin), which is a component of the EARP complex that is involved in endocytic recycling. It is required to tether the EARP complex to recycling endosomes. Nevertheless, it is involved in retrograde transport from early and late endosomes to the TGN [].
