Type |
Details |
Score |
Publication |
First Author: |
Nah J |
Year: |
2021 |
Journal: |
Exp Mol Med |
Title: |
The roles of the inhibitory autophagy regulator Rubicon in the heart: A new therapeutic target to prevent cardiac cell death. |
Volume: |
53 |
Issue: |
4 |
Pages: |
528-536 |
|
•
•
•
•
•
|
Publication |
First Author: |
Chang C |
Year: |
2019 |
Journal: |
Mol Cell |
Title: |
Bidirectional Control of Autophagy by BECN1 BARA Domain Dynamics. |
Volume: |
73 |
Issue: |
2 |
Pages: |
339-353.e6 |
|
•
•
•
•
•
|
Publication |
First Author: |
Cheng X |
Year: |
2017 |
Journal: |
Mol Cell |
Title: |
Pacer Mediates the Function of Class III PI3K and HOPS Complexes in Autophagosome Maturation by Engaging Stx17. |
Volume: |
65 |
Issue: |
6 |
Pages: |
1029-1043.e5 |
|
•
•
•
•
•
|
Publication |
First Author: |
Beltran S |
Year: |
2019 |
Journal: |
Mol Neurodegener |
Title: |
Network approach identifies Pacer as an autophagy protein involved in ALS pathogenesis. |
Volume: |
14 |
Issue: |
1 |
Pages: |
14 |
|
•
•
•
•
•
|
Protein Domain |
Type: |
Family |
Description: |
FAM98A, B and C are glycine-rich proteins found from worms to humans. FAM98A contains a tubulin-binding calponin homology domain. It interacts with PLEKHM1 and functions in lysosome positioning in osteoclasts []. FAM98A and FAM98B are included in a novel complex with DDX1 and C14orf166 and are involved in colorectal cancer progression []. |
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•
•
•
•
•
|
Protein Domain |
Type: |
Domain |
Description: |
This is the Rubicon homology domain (RH) characterised at the C-terminal of Rubicon, PLEKHM1 and Pacer, proteins that modulate late steps in autophagy [, ]. Rubicon (RUBCN) negatively regulates autophagy and endolysosomal trafficking by inhibiting PI3K complex II (PI3KC3-C2), which impairs autophagosome maturation process. Decrease in autophagy is associated to aging, then suppression of this process by Rubicon has been linked to decreased clearance of alpha-synuclein aggregates in neural tissues, impairment of liver cell homeostasis, and interstitial fibrosis in the kidney. PLEKHM1 is an adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways [, ], being involved in the suppression of endocytic transport rather than autophagosome maturation. Mutations in PLEKHM1 causes osteopetrosis []. On the other hand, Pacer (Protein associated with UVRAG as autophagy enhancer or Rubicon-like) positively regulates autophagy, promoting autophagosome maturation by facilitating the biogenesis of phosphatidylinositol 3-phosphate (PtdIns3P) in late steps of autophagy [, ]. It antagonizes RUBCN, thereby stimulating phosphatidylinositol 3-kinase activity of the PI3K/PI3KC3 complex []. Pacer is involved in neuronal autophagy, whose deficiency leads to impaired autophagy and accumulation of protein aggregates in ALS which correlates with cell death and vulnerability of motoneurons during ALS pathogenesis [].This domain contains nine conserved cysteines and one conserved histidine, which have been predicted to bind divalent zinc cations, being required for Rubicon and PLEKHM1 to interact with Rab7 [, ]. |
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•
•
•
•
•
|
Protein |
Organism: |
Mus musculus/domesticus |
Length: |
448
|
Fragment?: |
false |
|
•
•
•
•
•
|
Protein |
Organism: |
Mus musculus/domesticus |
Length: |
956
|
Fragment?: |
false |
|
•
•
•
•
•
|
Protein |
Organism: |
Mus musculus/domesticus |
Length: |
460
|
Fragment?: |
false |
|
•
•
•
•
•
|
Protein |
Organism: |
Mus musculus/domesticus |
Length: |
880
|
Fragment?: |
false |
|
•
•
•
•
•
|
Protein |
Organism: |
Mus musculus/domesticus |
Length: |
472
|
Fragment?: |
false |
|
•
•
•
•
•
|
Publication |
First Author: |
McEwan DG |
Year: |
2015 |
Journal: |
Autophagy |
Title: |
PLEKHM1: Adapting to life at the lysosome. |
Volume: |
11 |
Issue: |
4 |
Pages: |
720-2 |
|
•
•
•
•
•
|
Publication |
First Author: |
van Wesenbeeck L |
Year: |
2004 |
Journal: |
J Bone Miner Res |
Title: |
Localization of the gene causing the osteopetrotic phenotype in the incisors absent (ia) rat on chromosome 10q32.1. |
Volume: |
19 |
Issue: |
2 |
Pages: |
183-9 |
|
•
•
•
•
•
|
Protein Domain |
Type: |
Domain |
Description: |
PLEKHM1 is a ubiquitously expressed protein involved in the regulation of osteoclast function and bone resorption []. It may function as an adaptor protein that acts as a central hub to integrate endocytic and autophagic pathways at the lysosome []. PLEKHM1 contains an N-terminal RUN domain (RPIP8/RaP2 interacting protein 8, UNC-14 and NESCA/new molecule containing SH3 at the carboxyl-terminus), followed by a PH domain, and either a C1 domain or a DUF4206 domain at its C terminus. The RUN domain is thought to be involved in Rab-mediated membrane trafficking, possibly as a Rab-binding site. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner []. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity []. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane []. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes []. |
|
•
•
•
•
•
|
Protein |
Organism: |
Mus musculus/domesticus |
Length: |
761
|
Fragment?: |
false |
|
•
•
•
•
•
|
Protein |
Organism: |
Mus musculus/domesticus |
Length: |
1074
|
Fragment?: |
false |
|
•
•
•
•
•
|
Publication |
First Author: |
Lemmon MA |
Year: |
2004 |
Journal: |
Biochem Soc Trans |
Title: |
Pleckstrin homology domains: not just for phosphoinositides. |
Volume: |
32 |
Issue: |
Pt 5 |
Pages: |
707-11 |
|
•
•
•
•
•
|
Publication |
First Author: |
Cozier GE |
Year: |
2004 |
Journal: |
Curr Top Microbiol Immunol |
Title: |
Membrane targeting by pleckstrin homology domains. |
Volume: |
282 |
|
Pages: |
49-88 |
|
•
•
•
•
•
|
Publication |
First Author: |
Scheffzek K |
Year: |
2012 |
Journal: |
FEBS Lett |
Title: |
Pleckstrin homology (PH) like domains - versatile modules in protein-protein interaction platforms. |
Volume: |
586 |
Issue: |
17 |
Pages: |
2662-73 |
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•
•
•
•
•
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