| Type |
Details |
Score |
| Publication |
| First Author: |
Farini D |
| Year: |
2020 |
| Journal: |
Cell Rep |
| Title: |
A Dynamic Splicing Program Ensures Proper Synaptic Connections in the Developing Cerebellum. |
| Volume: |
31 |
| Issue: |
9 |
| Pages: |
107703 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Zhou L |
| Year: |
2017 |
| Journal: |
FASEB J |
| Title: |
Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development. |
| Volume: |
31 |
| Issue: |
1 |
| Pages: |
132-147 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Akagawa R |
| Year: |
2021 |
| Journal: |
Front Cell Dev Biol |
| Title: |
Alternative Functions of Cell Cycle-Related and DNA Repair Proteins in Post-mitotic Neurons. |
| Volume: |
9 |
|
| Pages: |
753175 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
GO Central curators, GOA curators, Rhea curators |
| Year: |
2020 |
|
| Title: |
Automatic Gene Ontology annotation based on Rhea mapping |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
GOA curators, MGI curators |
| Year: |
2001 |
|
| Title: |
Gene Ontology annotation based on Enzyme Commission mapping |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2005 |
|
| Title: |
Mouse Synonym Curation |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2003 |
|
| Title: |
Data Curation Using Mouse Genome Assembly |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Hansen J |
| Year: |
2003 |
| Journal: |
Proc Natl Acad Sci U S A |
| Title: |
A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome. |
| Volume: |
100 |
| Issue: |
17 |
| Pages: |
9918-22 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
The Gene Ontology Consortium |
| Year: |
2016 |
|
| Title: |
Automatic assignment of GO terms using logical inference, based on on inter-ontology links |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Stryke D |
| Year: |
2003 |
| Journal: |
Nucleic Acids Res |
| Title: |
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
| Volume: |
31 |
| Issue: |
1 |
| Pages: |
278-81 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
International Knockout Mouse Consortium |
| Year: |
2014 |
| Journal: |
Database Download |
| Title: |
MGI download of modified allele data from IKMC and creation of new knockout alleles |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Lennon G |
| Year: |
1999 |
| Journal: |
Database Download |
| Title: |
WashU-HHMI Mouse EST Project |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Velocigene |
| Year: |
2008 |
| Journal: |
MGI Direct Data Submission |
| Title: |
Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals) |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
International Mouse Strain Resource |
| Year: |
2014 |
| Journal: |
Database Download |
| Title: |
MGI download of germline transmission data for alleles from IMSR strain data |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Wellcome Trust Sanger Institute |
| Year: |
2010 |
| Journal: |
MGI Direct Data Submission |
| Title: |
Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics and the International Mouse Phenotyping Consortium (IMPC) |
| Year: |
2014 |
| Journal: |
Database Release |
| Title: |
Obtaining and Loading Phenotype Annotations from the International Mouse Phenotyping Consortium (IMPC) Database |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Hansen GM |
| Year: |
2008 |
| Journal: |
Genome Res |
| Title: |
Large-scale gene trapping in C57BL/6N mouse embryonic stem cells. |
| Volume: |
18 |
| Issue: |
10 |
| Pages: |
1670-9 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Carninci P |
| Year: |
2005 |
| Journal: |
Science |
| Title: |
The transcriptional landscape of the mammalian genome. |
| Volume: |
309 |
| Issue: |
5740 |
| Pages: |
1559-63 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Adams DJ |
| Year: |
2024 |
| Journal: |
Nature |
| Title: |
Genetic determinants of micronucleus formation in vivo. |
| Volume: |
627 |
| Issue: |
8002 |
| Pages: |
130-136 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
MGD Nomenclature Committee |
| Year: |
1995 |
|
| Title: |
Nomenclature Committee Use |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
GemPharmatech |
| Year: |
2020 |
|
| Title: |
GemPharmatech Website. |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Skarnes WC |
| Year: |
2011 |
| Journal: |
Nature |
| Title: |
A conditional knockout resource for the genome-wide study of mouse gene function. |
| Volume: |
474 |
| Issue: |
7351 |
| Pages: |
337-42 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI) |
| Year: |
2008 |
| Journal: |
Database Download |
| Title: |
Mouse Gene Trap Data Load from dbGSS |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
UniProt-GOA |
| Year: |
2012 |
|
| Title: |
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
GOA curators |
| Year: |
2016 |
|
| Title: |
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
The Jackson Laboratory Mouse Radiation Hybrid Database |
| Year: |
2004 |
| Journal: |
Database Release |
| Title: |
Mouse T31 Radiation Hybrid Data Load |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
The Gene Ontology Consortium |
| Year: |
2010 |
|
| Title: |
Automated transfer of experimentally-verified manual GO annotation data to mouse-human orthologs |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Diez-Roux G |
| Year: |
2011 |
| Journal: |
PLoS Biol |
| Title: |
A high-resolution anatomical atlas of the transcriptome in the mouse embryo. |
| Volume: |
9 |
| Issue: |
1 |
| Pages: |
e1000582 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2002 |
|
| Title: |
Mouse Genome Informatics Computational Sequence to Gene Associations |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2010 |
| Journal: |
Database Download |
| Title: |
Mouse Microarray Data Integration in Mouse Genome Informatics, the Affymetrix GeneChip Mouse Genome U74 Array Platform (A, B, C v2). |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
MGI Genome Annotation Group and UniGene Staff |
| Year: |
2015 |
| Journal: |
Database Download |
| Title: |
MGI-UniGene Interconnection Effort |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Marc Feuermann, Huaiyu Mi, Pascale Gaudet, Dustin Ebert, Anushya Muruganujan, Paul Thomas |
| Year: |
2010 |
|
| Title: |
Annotation inferences using phylogenetic trees |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Database and National Center for Biotechnology Information |
| Year: |
2000 |
| Journal: |
Database Release |
| Title: |
Entrez Gene Load |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Allen Institute for Brain Science |
| Year: |
2004 |
| Journal: |
Allen Institute |
| Title: |
Allen Brain Atlas: mouse riboprobes |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2009 |
| Journal: |
Database Download |
| Title: |
Mouse Microarray Data Integration in Mouse Genome Informatics, the Affymetrix GeneChip Mouse Gene 1.0 ST Array Platform |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics (MGI) and The National Center for Biotechnology Information (NCBI) |
| Year: |
2010 |
| Journal: |
Database Download |
| Title: |
Consensus CDS project |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Group |
| Year: |
2003 |
| Journal: |
Database Procedure |
| Title: |
Automatic Encodes (AutoE) Reference |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Bairoch A |
| Year: |
1999 |
| Journal: |
Database Release |
| Title: |
SWISS-PROT Annotated protein sequence database |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2005 |
|
| Title: |
Obtaining and Loading Genome Assembly Coordinates from Ensembl Annotations |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics |
| Year: |
2010 |
| Journal: |
Database Release |
| Title: |
Protein Ontology Association Load. |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2005 |
|
| Title: |
Obtaining and loading genome assembly coordinates from NCBI annotations |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2009 |
| Journal: |
Database Download |
| Title: |
Mouse Microarray Data Integration in Mouse Genome Informatics, the Affymetrix GeneChip Mouse Genome 430 2.0 Array Platform |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Kurita M |
| Year: |
2004 |
| Journal: |
Biochem Biophys Res Commun |
| Title: |
Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest. |
| Volume: |
324 |
| Issue: |
2 |
| Pages: |
554-61 |
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
40 |
| Description: |
Phosphoserine; by CDC7 |
| Type: |
modified residue |
| End: |
40 |
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
53 |
| Description: |
Phosphoserine; by CDC7 |
| Type: |
modified residue |
| End: |
53 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Li X |
| Year: |
2018 |
| Journal: |
Mol Cell |
| Title: |
Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. |
| Volume: |
72 |
| Issue: |
4 |
| Pages: |
650-660.e8 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Yamashita N |
| Year: |
2005 |
| Journal: |
Genes Cells |
| Title: |
Functional analyses of mouse ASK, an activation subunit for Cdc7 kinase, using conditional ASK knockout ES cells. |
| Volume: |
10 |
| Issue: |
6 |
| Pages: |
551-63 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Duncker BP |
| Year: |
2003 |
| Journal: |
Mutat Res |
| Title: |
Cdc7 kinases (DDKs) and checkpoint responses: lessons from two yeasts. |
| Volume: |
532 |
| Issue: |
1-2 |
| Pages: |
21-7 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Grishina I |
| Year: |
2005 |
| Journal: |
Cell Cycle |
| Title: |
A novel Cdk2 interactor is phosphorylated by Cdc7 and associates with components of the replication complexes. |
| Volume: |
4 |
| Issue: |
8 |
| Pages: |
1120-6 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Dowell SJ |
| Year: |
1994 |
| Journal: |
Science |
| Title: |
Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo. |
| Volume: |
265 |
| Issue: |
5176 |
| Pages: |
1243-6 |
|
•
•
•
•
•
|
| Allele |
| Name: |
transgene insertion DKO28, Hisao Masai |
| Allele Type: |
Transgenic |
| Attribute String: |
Inserted expressed sequence |
|
•
•
•
•
•
|
| Genotype |
| Symbol: |
Cdc7/Cdc7 Tg(EF1A-Cdc7)DKO28Hmas/? |
| Background: |
involves: 129S/Sv * C57BL/6 |
| Zygosity: |
cx |
| Has Mutant Allele: |
true |
|
•
•
•
•
•
|
| Genotype |
| Symbol: |
Cdc7/Cdc7 Tg(EF1A-Cdc7)DKO28Hmas/Tg(EF1A-Cdc7)DKO28Hmas |
| Background: |
involves: 129S/Sv * C57BL/6 |
| Zygosity: |
cx |
| Has Mutant Allele: |
true |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Homologous_superfamily |
| Description: |
In eukaryotes, initiation of DNA replication requires the assembly of pre-replication complexes (pre-RCs) on chromatin during the G1 phase. In the S phase, pre-RCs are activated by two protein kinases, Cdk2 and Cdc7, which results in the loading of replication factors and the unwinding of replication origins by the MCM helicase complex []. Cdc7 is a serine/threonine kinase that is conserved from yeast to human. It is regulated by its association with a regulatory subunit, the Dbf4 protein. This complex is often referred to as DDK (Dbf4-dependent kinase) [].DBF4 contains an N-terminal BRCT domain and a C-terminal conserved region that could potentially coordinate one zinc atom, the DBF4-type zinc finger. This entry represents the zinc finger, which is important for the interaction with Cdc7 [, ]. |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
212
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
118
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
116
 |
| Fragment?: |
true |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
262
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
322
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
145
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
139
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
71
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Family |
| Description: |
Cyclin-dependent kinase 2-interacting protein (CINP) is a component of theactive cyclin E/Cdk2 and cyclin A/Cdk2 complexes []. It is phopshorylated by Cdc7, but not by Cdk2 []. CINP has also been shown to bind to chromatin in a replication-dependent manner, and to associate with Origin Recognition Complex-2 (ORC2)-containing complexes and minichromosome maintenance/DNA replication licensing complex, MCM []. It has been proposed that CINP is part of the Cdc7-dependent mechanism of origin firing, and constitutes a physical link between Cdk2 and Cdc7 complexes at the origins []. |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Kurita M |
| Year: |
2007 |
| Journal: |
Biochem Biophys Res Commun |
| Title: |
CR/periphilin is a transcriptional co-repressor involved in cell cycle progression. |
| Volume: |
364 |
| Issue: |
4 |
| Pages: |
930-6 |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
114
 |
| Fragment?: |
true |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
2498
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Nasmyth K |
| Year: |
1996 |
| Journal: |
Science |
| Title: |
Viewpoint: putting the cell cycle in order. |
| Volume: |
274 |
| Issue: |
5293 |
| Pages: |
1643-5 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Hardy CF |
| Year: |
1996 |
| Journal: |
Mol Cell Biol |
| Title: |
A novel role for Cdc5p in DNA replication. |
| Volume: |
16 |
| Issue: |
12 |
| Pages: |
6775-82 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Ishimi Y |
| Year: |
2001 |
| Journal: |
J Biol Chem |
| Title: |
Biochemical activities associated with mouse Mcm2 protein. |
| Volume: |
276 |
| Issue: |
46 |
| Pages: |
42744-52 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Guo B |
| Year: |
2001 |
| Journal: |
Gene |
| Title: |
Cloning and characterization of Chinese hamster homologue of yeast DBF4 (ChDBF4). |
| Volume: |
264 |
| Issue: |
2 |
| Pages: |
249-56 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Wang C |
| Year: |
2019 |
| Journal: |
Nature |
| Title: |
Inducing and exploiting vulnerabilities for the treatment of liver cancer. |
| Volume: |
574 |
| Issue: |
7777 |
| Pages: |
268-272 |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
180
 |
| Fragment?: |
true |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Domain |
| Description: |
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [, , , , ]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domainsare often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both sequence and structure. They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. For example, Znf-containing proteins function in gene transcription, translation, mRNA trafficking, cytoskeleton organisation, epithelial development, cell adhesion, protein folding, chromatin remodelling and zinc sensing, to name but a few []. Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. In eukaryotes, initiation of DNA replication requires the assembly of pre-replication complexes (pre-RCs) on chromatin during the G1 phase. In the S phase, pre-RCs are activated by two protein kinases, Cdk2 and Cdc7, which results in the loading of replication factors and the unwinding of replication origins by the MCM helicase complex []. Cdc7 is a serine/threonine kinase that is conserved from yeast to human. It is regulated by its association with a regulatory subunit, the Dbf4 protein. This complex is often referred to as DDK (Dbf4-dependent kinase) [].DBF4 contains an N-terminal BRCT domain and a C-terminal conserved region that could potentially coordinate one zinc atom, the DBF4-type zinc finger. This entry represents the zinc finger, which is important for the interaction with Cdc7 [, ]. |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Kneissl M |
| Year: |
2003 |
| Journal: |
J Mol Biol |
| Title: |
Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells. |
| Volume: |
327 |
| Issue: |
1 |
| Pages: |
111-28 |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
663
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
663
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Family |
| Description: |
The MCM2-7 complex consists of six closely related proteins that are highly conserved throughout the eukaryotic kingdom. In eukaryotes, Mcm2 is a component of the MCM2-7 complex (MCM complex), which consists of six sequence-related AAA + type ATPases/helicases that form a hetero-hexameric ring []. MCM2-7 complex is part of the pre-replication complex (pre-RC). In G1 phase, inactive MCM2-7 complex is loaded onto origins of DNA replication [, , ]. During G1-S phase, MCM2-7 complex is activated to unwind the double stranded DNA and plays an important role in DNA replication forks elongation [].The components of the MCM2-7 complex are: DNA replication licensing factor MCM2, DNA replication licensing factor MCM3, DNA replication licensing factor MCM4, DNA replication licensing factor MCM5, DNA replication licensing factor MCM6, DNA replication licensing factor MCM7, In addition to its role in initiation of DNA replication, Mcm2 is able toinhibit the Mcm4,6,7 helicase. Studies on murine Mcm2 indicate that itsC terminus is required for interaction with MCM4, as well as for inhibitionof the DNA helicase activity of the Mcm4,6,7 complex. The N-terminal region,which contains an H3-binding domain and a region required for nuclear localisation, is required for the phosphorylation by CDC7 kinase. |
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| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
356
 |
| Fragment?: |
false |
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| Publication |
| First Author: |
Krapp A |
| Year: |
2008 |
| Journal: |
J Cell Sci |
| Title: |
Homoeostasis between the GTPase Spg1p and its GAP in the regulation of cytokinesis in S. pombe. |
| Volume: |
121 |
| Issue: |
Pt 5 |
| Pages: |
601-8 |
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| Publication |
| First Author: |
Cooper JA |
| Year: |
2006 |
| Journal: |
J Cell Biol |
| Title: |
Checkpoint control of mitotic exit--do budding yeast mind the GAP? |
| Volume: |
172 |
| Issue: |
3 |
| Pages: |
331-3 |
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| Publication |
| First Author: |
Schmidt S |
| Year: |
1997 |
| Journal: |
Genes Dev |
| Title: |
The Spg1p GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe. |
| Volume: |
11 |
| Issue: |
12 |
| Pages: |
1519-34 |
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| Protein Domain |
| Type: |
Family |
| Description: |
Small GTPases form an independent superfamily within the larger class of regulatory GTP hydrolases. This superfamily contains proteins that control a vast number of important processes and possess a common, structurally preserved GTP-binding domain [, ]. Sequence comparisons of small G proteins from various species have revealed that they are conserved in primary structures at the level of 30-55% similarity [].Crystallographic analysis of various small G proteins revealed the presence of a 20kDa catalytic domain that is unique for the whole superfamily [, ]. The domain is built of five alpha helices (A1-A5), six β-strands (B1-B6) and five polypeptide loops (G1-G5). A structural comparison of the GTP- and GDP-bound form, allows one to distinguish two functional loop regions: switch I and switch II that surround the gamma-phosphate group of the nucleotide. The G1 loop (also called the P-loop) that connects the B1 strand and the A1 helix is responsible for the binding of the phosphate groups. The G3 loop provides residues for Mg2 and phosphate binding and is located at the N terminus of the A2 helix. The G1 and G3 loops are sequentially similar to Walker A and Walker B boxes that are found in other nucleotide binding motifs. The G2 loop connects the A1 helix and the B2 strand and contains a conserved Thr residue responsible for Mg2 binding. The guanine base is recognised by the G4 and G5 loops. The consensus sequence NKXD of the G4 loop contains Lys and Asp residues directly interacting with the nucleotide. Part of the G5 loop located between B6 and A5 acts as a recognition site for the guanine base [].The small GTPase superfamily can be divided into at least 8 different families, including:Arf small GTPases. GTP-binding proteins involved in protein trafficking by modulating vesicle budding and uncoating within the Golgi apparatus.Ran small GTPases. GTP-binding proteins involved in nucleocytoplasmic transport. Required for the import of proteins into the nucleus and also for RNA export.Rab small GTPases. GTP-binding proteins involved in vesicular traffic.Rho small GTPases. GTP-binding proteins that control cytoskeleton reorganisation.Ras small GTPases. GTP-binding proteins involved in signalling pathways.Sar1 small GTPases. Small GTPase component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER).Mitochondrial Rho (Miro). Small GTPase domain found in mitochondrial proteins involved in mitochondrial trafficking.Roc small GTPases domain. Small GTPase domain always found associated with the COR domain.This entry includes Tem1 from budding yeasts and Spg1 from fission yeasts. They are GTPases involved in the regulation of the cell cycle. In Schizosaccharomyces pombe, Spg1 is required for the localisation of Cdc7 (part of the septation initiation network) to the spindle pole body (SPB) []. It is regulated negatively by a GTPase-activating protein (GAP) comprising two subunits - Byr4 and Cdc16. In anaphase B, Spg1 is localised on the new SPB []. In Saccharomyces cerevisiae, Tem1 is associated with the mitotic exit network (MEN). It is involved in termination of M phase of the cell cycle []. |
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| Publication |
| First Author: |
Remus D |
| Year: |
2009 |
| Journal: |
Cell |
| Title: |
Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing. |
| Volume: |
139 |
| Issue: |
4 |
| Pages: |
719-30 |
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| Publication |
| First Author: |
Evrin C |
| Year: |
2009 |
| Journal: |
Proc Natl Acad Sci U S A |
| Title: |
A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication. |
| Volume: |
106 |
| Issue: |
48 |
| Pages: |
20240-5 |
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| Publication |
| First Author: |
Symeonidou IE |
| Year: |
2012 |
| Journal: |
FEBS Lett |
| Title: |
Control over DNA replication in time and space. |
| Volume: |
586 |
| Issue: |
18 |
| Pages: |
2803-12 |
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| Publication |
| First Author: |
Singleton MR |
| Year: |
2007 |
| Journal: |
Annu Rev Biochem |
| Title: |
Structure and mechanism of helicases and nucleic acid translocases. |
| Volume: |
76 |
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| Pages: |
23-50 |
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| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
904
 |
| Fragment?: |
false |
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| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
913
 |
| Fragment?: |
false |
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| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
904
 |
| Fragment?: |
false |
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| Publication |
| First Author: |
Valencia A |
| Year: |
1991 |
| Journal: |
Biochemistry |
| Title: |
The ras protein family: evolutionary tree and role of conserved amino acids. |
| Volume: |
30 |
| Issue: |
19 |
| Pages: |
4637-48 |
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| Publication |
| First Author: |
Pai EF |
| Year: |
1990 |
| Journal: |
EMBO J |
| Title: |
Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis. |
| Volume: |
9 |
| Issue: |
8 |
| Pages: |
2351-9 |
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| Publication |
| First Author: |
Bourne HR |
| Year: |
1990 |
| Journal: |
Nature |
| Title: |
The GTPase superfamily: a conserved switch for diverse cell functions. |
| Volume: |
348 |
| Issue: |
6297 |
| Pages: |
125-32 |
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| Publication |
| First Author: |
Bourne HR |
| Year: |
1991 |
| Journal: |
Nature |
| Title: |
The GTPase superfamily: conserved structure and molecular mechanism. |
| Volume: |
349 |
| Issue: |
6305 |
| Pages: |
117-27 |
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| Publication |
| First Author: |
Paduch M |
| Year: |
2001 |
| Journal: |
Acta Biochim Pol |
| Title: |
Structure of small G proteins and their regulators. |
| Volume: |
48 |
| Issue: |
4 |
| Pages: |
829-50 |
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| Publication |
| First Author: |
Matthews JM |
| Year: |
2002 |
| Journal: |
IUBMB Life |
| Title: |
Zinc fingers--folds for many occasions. |
| Volume: |
54 |
| Issue: |
6 |
| Pages: |
351-5 |
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| Publication |
| First Author: |
Gamsjaeger R |
| Year: |
2007 |
| Journal: |
Trends Biochem Sci |
| Title: |
Sticky fingers: zinc-fingers as protein-recognition motifs. |
| Volume: |
32 |
| Issue: |
2 |
| Pages: |
63-70 |
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| Publication |
| First Author: |
Hall TM |
| Year: |
2005 |
| Journal: |
Curr Opin Struct Biol |
| Title: |
Multiple modes of RNA recognition by zinc finger proteins. |
| Volume: |
15 |
| Issue: |
3 |
| Pages: |
367-73 |
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| Publication |
| First Author: |
Brown RS |
| Year: |
2005 |
| Journal: |
Curr Opin Struct Biol |
| Title: |
Zinc finger proteins: getting a grip on RNA. |
| Volume: |
15 |
| Issue: |
1 |
| Pages: |
94-8 |
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| Publication |
| First Author: |
Klug A |
| Year: |
1999 |
| Journal: |
J Mol Biol |
| Title: |
Zinc finger peptides for the regulation of gene expression. |
| Volume: |
293 |
| Issue: |
2 |
| Pages: |
215-8 |
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| Publication |
| First Author: |
Laity JH |
| Year: |
2001 |
| Journal: |
Curr Opin Struct Biol |
| Title: |
Zinc finger proteins: new insights into structural and functional diversity. |
| Volume: |
11 |
| Issue: |
1 |
| Pages: |
39-46 |
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