| Type |
Details |
Score |
| GXD Expression |
| Probe: |
MGI:1765085 |
| Assay Type: |
RNA in situ |
| Annotation Date: |
2010-09-14 |
| Strength: |
Moderate |
| Sex: |
Not Specified |
| Emaps: |
EMAPS:1768023 |
| Pattern: |
Regionally restricted |
| Stage: |
TS23 |
| Assay Id: |
MGI:4827275 |
| Age: |
embryonic day 14.5 |
| Image: |
euxassay_003979_02 |
|
| Specimen Label: |
euxassay_003979_02 |
| Detected: |
true |
| Specimen Num: |
2 |
|
•
•
•
•
•
|
| GXD Expression |
| Probe: |
MGI:1765085 |
| Assay Type: |
RNA in situ |
| Annotation Date: |
2010-09-14 |
| Strength: |
Moderate |
| Sex: |
Not Specified |
| Emaps: |
EMAPS:1768023 |
| Pattern: |
Regionally restricted |
| Stage: |
TS23 |
| Assay Id: |
MGI:4827275 |
| Age: |
embryonic day 14.5 |
| Image: |
euxassay_003979_03 |
|
| Specimen Label: |
euxassay_003979_03 |
| Detected: |
true |
| Specimen Num: |
3 |
|
•
•
•
•
•
|
| GXD Expression |
| Probe: |
MGI:1765085 |
| Assay Type: |
RNA in situ |
| Annotation Date: |
2010-09-14 |
| Strength: |
Moderate |
| Sex: |
Not Specified |
| Emaps: |
EMAPS:1768023 |
| Pattern: |
Regionally restricted |
| Stage: |
TS23 |
| Assay Id: |
MGI:4827275 |
| Age: |
embryonic day 14.5 |
| Image: |
euxassay_003979_04 |
|
| Specimen Label: |
euxassay_003979_04 |
| Detected: |
true |
| Specimen Num: |
4 |
|
•
•
•
•
•
|
| GXD Expression |
| Probe: |
MGI:1765085 |
| Assay Type: |
RNA in situ |
| Annotation Date: |
2010-09-14 |
| Strength: |
Moderate |
| Sex: |
Not Specified |
| Emaps: |
EMAPS:1768023 |
| Pattern: |
Regionally restricted |
| Stage: |
TS23 |
| Assay Id: |
MGI:4827275 |
| Age: |
embryonic day 14.5 |
| Image: |
euxassay_003979_05 |
|
| Specimen Label: |
euxassay_003979_05 |
| Detected: |
true |
| Specimen Num: |
5 |
|
•
•
•
•
•
|
| GXD Expression |
| Probe: |
MGI:1765085 |
| Assay Type: |
RNA in situ |
| Annotation Date: |
2010-09-14 |
| Strength: |
Moderate |
| Sex: |
Not Specified |
| Emaps: |
EMAPS:1768023 |
| Pattern: |
Regionally restricted |
| Stage: |
TS23 |
| Assay Id: |
MGI:4827275 |
| Age: |
embryonic day 14.5 |
| Image: |
euxassay_003979_06 |
|
| Specimen Label: |
euxassay_003979_06 |
| Detected: |
true |
| Specimen Num: |
6 |
|
•
•
•
•
•
|
| GXD Expression |
| Probe: |
MGI:1765085 |
| Assay Type: |
RNA in situ |
| Annotation Date: |
2010-09-14 |
| Strength: |
Moderate |
| Sex: |
Not Specified |
| Emaps: |
EMAPS:1768023 |
| Pattern: |
Regionally restricted |
| Stage: |
TS23 |
| Assay Id: |
MGI:4827275 |
| Age: |
embryonic day 14.5 |
| Image: |
euxassay_003979_23 |
|
| Specimen Label: |
euxassay_003979_23 |
| Detected: |
true |
| Specimen Num: |
18 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Sladky VC |
| Year: |
2022 |
| Journal: |
Genes Dev |
| Title: |
Centriole signaling restricts hepatocyte ploidy to maintain liver integrity. |
| Volume: |
36 |
| Issue: |
13-14 |
| Pages: |
843-56 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Marthiens V |
| Year: |
2013 |
| Journal: |
Nat Cell Biol |
| Title: |
Centrosome amplification causes microcephaly. |
| Volume: |
15 |
| Issue: |
7 |
| Pages: |
731-40 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Vulprecht J |
| Year: |
2012 |
| Journal: |
J Cell Sci |
| Title: |
STIL is required for centriole duplication in human cells. |
| Volume: |
125 |
| Issue: |
Pt 5 |
| Pages: |
1353-62 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Zhu F |
| Year: |
2008 |
| Journal: |
Curr Biol |
| Title: |
The mammalian SPD-2 ortholog Cep192 regulates centrosome biogenesis. |
| Volume: |
18 |
| Issue: |
2 |
| Pages: |
136-41 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mahjoub MR |
| Year: |
2012 |
| Journal: |
Curr Biol |
| Title: |
Supernumerary centrosomes nucleate extra cilia and compromise primary cilium signaling. |
| Volume: |
22 |
| Issue: |
17 |
| Pages: |
1628-34 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Weiss J |
| Year: |
2012 |
| Journal: |
Mamm Genome |
| Title: |
ENU mutagenesis in mice identifies candidate genes for hypogonadism. |
| Volume: |
23 |
| Issue: |
5-6 |
| Pages: |
346-55 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Jordan PW |
| Year: |
2012 |
| Journal: |
J Cell Sci |
| Title: |
Polo-like kinase is required for synaptonemal complex disassembly and phosphorylation in mouse spermatocytes. |
| Volume: |
125 |
| Issue: |
Pt 21 |
| Pages: |
5061-72 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Hudson JW |
| Year: |
2000 |
| Journal: |
Gene |
| Title: |
Sak kinase gene structure and transcriptional regulation. |
| Volume: |
241 |
| Issue: |
1 |
| Pages: |
65-73 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Marjanović M |
| Year: |
2015 |
| Journal: |
Nat Commun |
| Title: |
CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination. |
| Volume: |
6 |
|
| Pages: |
7676 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Goetz JJ |
| Year: |
2016 |
| Journal: |
PLoS One |
| Title: |
Polo-Like Kinase 3 Appears Dispensable for Normal Retinal Development Despite Robust Embryonic Expression. |
| Volume: |
11 |
| Issue: |
3 |
| Pages: |
e0150878 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Carninci P |
| Year: |
2005 |
| Journal: |
Science |
| Title: |
The transcriptional landscape of the mammalian genome. |
| Volume: |
309 |
| Issue: |
5740 |
| Pages: |
1559-63 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Freeman TC |
| Year: |
1998 |
| Journal: |
MGI Direct Data Submission |
| Title: |
Expression Mapping of Mouse Genes |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Birgit Meldal and Sandra Orchard (1). (1) European Bioinformatics Institute (EBI), Hinxton, Cambridgeshire, United Kingdom |
| Year: |
2023 |
|
| Title: |
Manual transfer of experimentally-verified manual GO annotation data to homologous complexes by curator judgment of sequence, composition and function similarity |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
GO Central curators, GOA curators, Rhea curators |
| Year: |
2020 |
|
| Title: |
Automatic Gene Ontology annotation based on Rhea mapping |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Stryke D |
| Year: |
2003 |
| Journal: |
Nucleic Acids Res |
| Title: |
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
| Volume: |
31 |
| Issue: |
1 |
| Pages: |
278-81 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Bedogni F |
| Year: |
2021 |
| Journal: |
Front Mol Neurosci |
| Title: |
Cell-Type-Specific Gene Expression in Developing Mouse Neocortex: Intermediate Progenitors Implicated in Axon Development. |
| Volume: |
14 |
|
| Pages: |
686034 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Lennon G |
| Year: |
1999 |
| Journal: |
Database Download |
| Title: |
WashU-HHMI Mouse EST Project |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Wellcome Trust Sanger Institute |
| Year: |
2009 |
| Journal: |
MGI Direct Data Submission |
| Title: |
Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
UniProt-GOA |
| Year: |
2012 |
|
| Title: |
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2002 |
|
| Title: |
Chromosome assignment of mouse genes using the Mouse Genome Sequencing Consortium (MGSC) assembly and the ENSEMBL Database |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Kawai J |
| Year: |
2001 |
| Journal: |
Nature |
| Title: |
Functional annotation of a full-length mouse cDNA collection. |
| Volume: |
409 |
| Issue: |
6821 |
| Pages: |
685-90 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
MGD Nomenclature Committee |
| Year: |
1995 |
|
| Title: |
Nomenclature Committee Use |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
GemPharmatech |
| Year: |
2020 |
|
| Title: |
GemPharmatech Website. |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Skarnes WC |
| Year: |
2011 |
| Journal: |
Nature |
| Title: |
A conditional knockout resource for the genome-wide study of mouse gene function. |
| Volume: |
474 |
| Issue: |
7351 |
| Pages: |
337-42 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI) |
| Year: |
2008 |
| Journal: |
Database Download |
| Title: |
Mouse Gene Trap Data Load from dbGSS |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
AgBase, BHF-UCL, Parkinson's UK-UCL, dictyBase, HGNC, Roslin Institute, FlyBase and UniProtKB curators |
| Year: |
2011 |
|
| Title: |
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
UniProt-GOA |
| Year: |
2012 |
|
| Title: |
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
GOA curators |
| Year: |
2016 |
|
| Title: |
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
The Jackson Laboratory Mouse Radiation Hybrid Database |
| Year: |
2004 |
| Journal: |
Database Release |
| Title: |
Mouse T31 Radiation Hybrid Data Load |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Okazaki Y |
| Year: |
2002 |
| Journal: |
Nature |
| Title: |
Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. |
| Volume: |
420 |
| Issue: |
6915 |
| Pages: |
563-73 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
The Gene Ontology Consortium |
| Year: |
2010 |
|
| Title: |
Automated transfer of experimentally-verified manual GO annotation data to mouse-human orthologs |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Diez-Roux G |
| Year: |
2011 |
| Journal: |
PLoS Biol |
| Title: |
A high-resolution anatomical atlas of the transcriptome in the mouse embryo. |
| Volume: |
9 |
| Issue: |
1 |
| Pages: |
e1000582 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2002 |
|
| Title: |
Mouse Genome Informatics Computational Sequence to Gene Associations |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2010 |
| Journal: |
Database Download |
| Title: |
Mouse Microarray Data Integration in Mouse Genome Informatics, the Affymetrix GeneChip Mouse Genome U74 Array Platform (A, B, C v2). |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
MGI Genome Annotation Group and UniGene Staff |
| Year: |
2015 |
| Journal: |
Database Download |
| Title: |
MGI-UniGene Interconnection Effort |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Marc Feuermann, Huaiyu Mi, Pascale Gaudet, Dustin Ebert, Anushya Muruganujan, Paul Thomas |
| Year: |
2010 |
|
| Title: |
Annotation inferences using phylogenetic trees |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Database and National Center for Biotechnology Information |
| Year: |
2000 |
| Journal: |
Database Release |
| Title: |
Entrez Gene Load |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Allen Institute for Brain Science |
| Year: |
2004 |
| Journal: |
Allen Institute |
| Title: |
Allen Brain Atlas: mouse riboprobes |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2009 |
| Journal: |
Database Download |
| Title: |
Mouse Microarray Data Integration in Mouse Genome Informatics, the Affymetrix GeneChip Mouse Gene 1.0 ST Array Platform |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics (MGI) and The National Center for Biotechnology Information (NCBI) |
| Year: |
2010 |
| Journal: |
Database Download |
| Title: |
Consensus CDS project |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Group |
| Year: |
2003 |
| Journal: |
Database Procedure |
| Title: |
Automatic Encodes (AutoE) Reference |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Bairoch A |
| Year: |
1999 |
| Journal: |
Database Release |
| Title: |
SWISS-PROT Annotated protein sequence database |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2005 |
|
| Title: |
Obtaining and Loading Genome Assembly Coordinates from Ensembl Annotations |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics |
| Year: |
2010 |
| Journal: |
Database Release |
| Title: |
Protein Ontology Association Load. |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2005 |
|
| Title: |
Obtaining and loading genome assembly coordinates from NCBI annotations |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Mouse Genome Informatics Scientific Curators |
| Year: |
2009 |
| Journal: |
Database Download |
| Title: |
Mouse Microarray Data Integration in Mouse Genome Informatics, the Affymetrix GeneChip Mouse Genome 430 2.0 Array Platform |
|
|
|
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
372 |
| Description: |
Phosphoserine; by PLK4 |
| Type: |
modified residue |
| End: |
372 |
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
1 |
| Description: |
Serine/threonine-protein kinase PLK4 |
| Type: |
chain |
| End: |
925 |
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
107 |
| Description: |
Phosphothreonine; by PLK4 |
| Type: |
modified residue |
| End: |
107 |
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
109 |
| Description: |
Phosphoserine; by PLK4 |
| Type: |
modified residue |
| End: |
109 |
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
151 |
| Description: |
Phosphoserine; by PLK4 |
| Type: |
modified residue |
| End: |
151 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
LoMastro GM |
| Year: |
2022 |
| Journal: |
bioRxiv |
| Title: |
PLK4 drives centriole amplification and apical surface area expansion in multiciliated cells |
|
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Yang XD |
| Year: |
2020 |
| Journal: |
EMBO J |
| Title: |
PLK4 deubiquitination by Spata2-CYLD suppresses NEK7-mediated NLRP3 inflammasome activation at the centrosome. |
| Volume: |
39 |
| Issue: |
2 |
| Pages: |
e102201 |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
464
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
898
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| UniProt Feature |
| Begin: |
78 |
| Description: |
Phosphoserine; by MAPKAPK2 and PLK4 |
| Type: |
modified residue |
| End: |
78 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Cizmecioglu O |
| Year: |
2010 |
| Journal: |
J Cell Biol |
| Title: |
Cep152 acts as a scaffold for recruitment of Plk4 and CPAP to the centrosome. |
| Volume: |
191 |
| Issue: |
4 |
| Pages: |
731-9 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Liu Y |
| Year: |
2018 |
| Journal: |
Nat Commun |
| Title: |
Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly. |
| Volume: |
9 |
| Issue: |
1 |
| Pages: |
1731 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Shimanovskaya E |
| Year: |
2014 |
| Journal: |
Structure |
| Title: |
Structure of the C. elegans ZYG-1 cryptic polo box suggests a conserved mechanism for centriolar docking of Plk4 kinases. |
| Volume: |
22 |
| Issue: |
8 |
| Pages: |
1090-1104 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Leung GC |
| Year: |
2002 |
| Journal: |
Nat Struct Biol |
| Title: |
The Sak polo-box comprises a structural domain sufficient for mitotic subcellular localization. |
| Volume: |
9 |
| Issue: |
10 |
| Pages: |
719-24 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Puklowski A |
| Year: |
2011 |
| Journal: |
Nat Cell Biol |
| Title: |
The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplication. |
| Volume: |
13 |
| Issue: |
8 |
| Pages: |
1004-9 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Bahtz R |
| Year: |
2012 |
| Journal: |
J Cell Sci |
| Title: |
GCP6 is a substrate of Plk4 and required for centriole duplication. |
| Volume: |
125 |
| Issue: |
Pt 2 |
| Pages: |
486-96 |
|
•
•
•
•
•
|
| DO Term |
|
•
•
•
•
•
|
| Allele |
| Name: |
transgene insertion 39, Don W Cleveland |
| Allele Type: |
Transgenic |
| Attribute String: |
Conditional ready, Inserted expressed sequence, Reporter |
|
•
•
•
•
•
|
| Allele |
| Name: |
collagen, type I, alpha 1; targeted mutation 1, Andrew Holland |
| Allele Type: |
Targeted |
| Attribute String: |
Inducible, Inserted expressed sequence, Reporter |
|
•
•
•
•
•
|
| Strain |
| Attribute String: |
coisogenic, mutant strain, transgenic |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Vitre B |
| Year: |
2015 |
| Journal: |
Proc Natl Acad Sci U S A |
| Title: |
Chronic centrosome amplification without tumorigenesis. |
| Volume: |
112 |
| Issue: |
46 |
| Pages: |
E6321-30 |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Family |
| Description: |
Cep85 is a centrosomal protein of 85kDa []. It is a centriole duplication factor that directly interacts with STIL. It is essential for efficient centriolar targeting of STIL, PLK4 activation and faithful daughter centriole assembly []. |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Domain |
| Description: |
In metazoans, Plk4 kinases control daughter centriole assembly. Plk4 homologues have an N-terminal kinase domain, a C-terminal polo box, and a central domain termed the 'cryptic polo box' (CPB) that has been shown to dimerize, to be sufficient for centriole localization and to be required for Plk4 to promote centriole assembly. Probable serine/threonine-protein kinase zyg-1 () (ZYG-1) is a Plk4 homlogue found in C. elegans. Crystal structure for the CPB of C. elegans ZYG-1, reveals that it forms a Z-shaped dimer containing an intermolecular β-sheet with an extended basic surface patch. Electrostatic interactions between the basic patch on the ZYG-1 CPB dimer and the SPD-2 acidic region dock ZYG-1 onto centrioles to promote new centriole assembly. ZYG-1 CPB contains two tandem polo boxes (PB1 and PB2), each containing a six-stranded β-sheet with an α-helix packed against one side []. This entry represents PB1. |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Domain |
| Description: |
In metazoans, Plk4 kinases control daughter centriole assembly. Plk4 homologs have an N-terminal kinase domain, a C-terminal polo box, and a central domain termed the 'cryptic polo box' (CPB) that has been shown to dimerize, to be sufficient for centriole localization and to be required for Plk4 to promote centriole assembly. Probable serine/threonine-protein kinase zyg-1 () (ZYG-1) is a Plk4 homlog found in C. elegans. Crystal structure for the CPB of C. elegans ZYG-1, reveals that it forms a Z-shaped dimer containing an intermolecular β-sheet with an extended basic surface patch. Electrostatic interactions between the basic patch on the ZYG-1 CPB dimer and the SPD-2 acidic region dock ZYG-1 onto centrioles to promote new centriole assembly. ZYG-1 CPB contains two tandem polo boxes (PB1 and PB2), each containing a six-stranded β-sheet with an α-helix packed against one side []. This entry represents PB2. |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
1736
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Dzhindzhev NS |
| Year: |
2010 |
| Journal: |
Nature |
| Title: |
Asterless is a scaffold for the onset of centriole assembly. |
| Volume: |
467 |
| Issue: |
7316 |
| Pages: |
714-8 |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Family |
| Description: |
Centrosomal protein of 152kDa (Cep152) acts as a scaffold for recruitment of centriole regulators, Plk4 and CPAP, to the centrosome [, ]. In multiciliated cell (MCCs), the basal bodies of the motile cilia are derived from centroles, which are amplified in a structure known as the deuterostome. Cep152 is required for the centriole biogenesis in deuterostome []. |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Homologous_superfamily |
| Description: |
The polo-like Ser/Thr kinases (Plk1, Plk2/Snk, Plk3/Prk/Fnk, Plk4/Sak, and the inactive kinase Plk5) play various roles in cytokinesis and mitosis. At their C terminus, they contain a tandemly repeated polo-box domain (PBD) (in the case of Plk4, a tandem repeat of cryptic PBDs is found in the middle of the protein followed by a C-terminal single repeat), which appears to be involved in autoinhibition and in mediating the subcellular localization. The latter may be controlled via interactions between the polo-box domain and phospho-peptide motifs. The phosphopeptide binding site is formed at the interface between the two tandemly repeated PBDs. The PBDs of Plk4/Sak appear unique in participating in homodimer interactions, though it is not clear whether and how they interact with phosphopeptides [, , , , , ].In metazoans, Plk4 kinases control daughter centriole assembly. Plk4 homologues have an N-terminal kinase domain, a C-terminal polo box, and a central domain termed the 'cryptic polo box' (CPB) that has been shown to dimerize, to be sufficient for centriole localization and to be required for Plk4 to promote centriole assembly. Probable serine/threonine-protein kinase zyg-1 () (ZYG-1) is a Plk4 homologue found in C. elegans. Crystal structure for the CPB of C. elegans ZYG-1, reveals that it forms a Z-shaped dimer containing an intermolecular β-sheet with an extended basic surface patch. Electrostatic interactions between the basic patch on the ZYG-1 CPB dimer and the SPD-2 acidic region dock ZYG-1 onto centrioles to promote new centriole assembly. ZYG-1 CPB contains two tandem polo boxes (PB1 and PB2), each containing a six-stranded β-sheet with an α-helix packed against one side [].This entry represents the first (cryptic) polo-box domain of Plk4 and homologues. |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
761
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
77
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
759
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
135
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Holland AJ |
| Year: |
2012 |
| Journal: |
Genes Dev |
| Title: |
The autoregulated instability of Polo-like kinase 4 limits centrosome duplication to once per cell cycle. |
| Volume: |
26 |
| Issue: |
24 |
| Pages: |
2684-9 |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Family |
| Description: |
Centriole amplification is controlled by two duplicated genes, Cep63 (centrosomal protein of 63kDa) and Deup1 (Deuterosome assembly protein 1).Cep63 regulates mother-centriole-dependent centriole duplication, whereas Deup1 is involved in large-scale de novo centriole biogenesis. Deup1, previously known as coiled-coil domain-containing protein 67, is a structural component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells. Deup1 binds to Cep152 and then recruits Plk4 to activate centriole biogenesis []. |
|
•
•
•
•
•
|
| Protein Domain |
| Type: |
Family |
| Description: |
This entry includes Cep85 and Cep85L from animals.Cep85 is a centrosomal protein of 85kDa []. It is a centriole duplication factor that directly interacts with STIL. It is essential for efficient centriolar targeting of STIL, PLK4 activation and faithful daughter centriole assembly [].Centrosomal protein of 85kDa-like (CEP85L) may localise to the centrosome []. It is also known as breast cancer antigen NY-BR-15 []. A chromosomal aberration involving CEP85L was found in a patient with T-lymphoblastic lymphoma and associated myeloproliferative neoplasm []. |
|
•
•
•
•
•
|
| Strain |
| Attribute String: |
congenic, mutant strain, targeted mutation |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
601
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
397
 |
| Fragment?: |
true |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
351
 |
| Fragment?: |
true |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
536
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Kelleher FC |
| Year: |
2019 |
| Journal: |
Carcinogenesis |
| Title: |
Targeting the centrosome and polo-like kinase 4 in osteosarcoma. |
| Volume: |
40 |
| Issue: |
4 |
| Pages: |
493-499 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Grzonka M |
| Year: |
2024 |
| Journal: |
Elife |
| Title: |
Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells. |
| Volume: |
13 |
|
|
|
•
•
•
•
•
|
| Publication |
| First Author: |
Levine MS |
| Year: |
2017 |
| Journal: |
Dev Cell |
| Title: |
Centrosome Amplification Is Sufficient to Promote Spontaneous Tumorigenesis in Mammals. |
| Volume: |
40 |
| Issue: |
3 |
| Pages: |
313-322.e5 |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
806
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
449
 |
| Fragment?: |
true |
|
•
•
•
•
•
|
| Protein |
| Organism: |
Mus musculus/domesticus |
| Length: |
704
 |
| Fragment?: |
false |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Scanlan MJ |
| Year: |
2001 |
| Journal: |
Cancer Immun |
| Title: |
Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression. |
| Volume: |
1 |
|
| Pages: |
4 |
|
•
•
•
•
•
|
| Publication |
| First Author: |
Braun VZ |
| Year: |
2024 |
| Journal: |
Sci Adv |
| Title: |
Extra centrosomes delay DNA damage-driven tumorigenesis. |
| Volume: |
10 |
| Issue: |
13 |
| Pages: |
eadk0564 |
|
•
•
•
•
•
|