Sld7 interacts with Sld3 and is required for the proper function of Sld3 at the initiation of DNA replication []. The origin association of Sld3, Sld7, and Cdc45 is the key to determining the temporal order of origin firing []. Sld7 is also required for mitochondrial morphology [].
Geminin coiled-coil domain-containing protein 1 (GEMC1) is a regulator of DNA replication. It promotes initiation of chromosomal DNA replication by mediating TopBP1- and cdk2-dependent recruitment of cdc45 onto replication origins []. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein. However, only some of the amino acid residues critical for geminin function are conserved in GEMC1 [].