| Primary Identifier | MGI:5902138 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rhox13 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele is derived from the "knockout first" vector used to generate the Rhox13 allele. The vector is constructed as follows. The L1L2_Bact_P cassette was inserted at position 38122599 of Chromosome X upstream of exon 2 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by the neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 38123363. Exon 2 is thus flanked by loxP sites. A conditional-ready (floxed) allele was created by subsequent flp-mediated recombination which removes the lacZ and neo cassettes, leaving exon 2 floxed. Finally, exon 2 was deleted through cre-mediated recombination, creating a null allele. |
