| First Author | Fukagawa T | Year | 1999 |
| Journal | Nucleic Acids Res | Volume | 27 |
| Issue | 9 | Pages | 1966-9 |
| PubMed ID | 10198428 | Mgi Jnum | J:54484 |
| Mgi Id | MGI:1335958 | Doi | 10.1093/nar/27.9.1966 |
| Citation | Fukagawa T, et al. (1999) The chicken HPRT gene: a counter selectable marker for the DT40 cell line. Nucleic Acids Res 27(9):1966-9 |
| abstractText | We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering. |
