| First Author | Mohati SM | Year | 2024 |
| Journal | STAR Protoc | Volume | 5 |
| Issue | 3 | Pages | 103208 |
| PubMed ID | 39068659 | Mgi Jnum | J:359089 |
| Mgi Id | MGI:7782737 | Doi | 10.1016/j.xpro.2024.103208 |
| Citation | Mohati SM, et al. (2024) A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cells. STAR Protoc 5(3):103208 |
| abstractText | Protein kinase C-delta (PKC-delta) is a key enzyme controlling growth, differentiation, and apoptosis in various cells, including immune cells. Here, we present a protocol to determine PKC-delta activation in response to increased membrane-bound diacylglycerol or phorbol-12-myristate-13-acetate treatment in murine bone-marrow-derived dendritic cells. We describe steps for dendritic cell differentiation, the isolation of plasma membrane lipids, and the quantification of diacylglycerol. We then detail procedures for measuring PKC-delta kinase activity by in vitro assay, indirect immunofluorescence, and western blotting experiments. For complete details on the use and execution of this protocol, please refer to Parsons et al.(1). |
