| Primary Identifier | MGI:7276202 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Mlh3 |
| Strain of Origin | FVB/NJ x B6(Cg)-Tyr<c-2J>/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using an sgRNA (targeting GTCAGCTAAGGGCAGCATTG) and an ssODN template (GAAGAGAGCTGCCGCCTCATCGAAGCTCTGTCCTTGTCCCAGCTGCCATTTCAGTGTACTCATGGGAGACCATCTATGCTGCCCTTAGCTGACCTGGACCACTTGGAGCAGGAAAAACAG) with CRISPR/Cas9 technology, alanine codon 1356 (GCT) was changed to threonine (ACT) (c.4066G>A, p.A1356T). This is the equivalent of the human p.A1394T mutation. |
