| First Author | Weber P | Year | 2003 |
| Journal | Biol Reprod | Volume | 68 |
| Issue | 2 | Pages | 553-9 |
| PubMed ID | 12533419 | Mgi Jnum | J:81392 |
| Mgi Id | MGI:2449243 | Doi | 10.1095/biolreprod.102.005801 |
| Citation | Weber P, et al. (2003) Temporally controlled site-specific mutagenesis in the germ cell lineage of the mouse testis. Biol Reprod 68(2):553-9 |
| abstractText | We have obtained a PrP-Cre-ER(T) transgenic mouse line (28.8) that selectively expresses in testis the tamoxifen-inducible Cre-ER(T) recombinase under the control of a mouse Prion protein (PrP) promoter-containing genomic fragment. Cre-ER(T) is expressed in spermatogonia and spermatocytes, but not in Sertoli and Leydig cells. We also established reporter PrP-L-EGFP-L transgenic mice harboring a LoxP-flanked enhanced green fluorescent protein (EGFP) Cre reporter cassette under the control of the same PrP promoter-containing genomic fragment that exhibits prominent EGFP expression in brain and testis. Using the PrP-L-EGFP-L as well as other Cre-reporter mice, we demonstrate that tamoxifen administration efficiently and selectively induces Cre-mediated recombination in the germ cell lineage. The established PrP-Cre-ER(T) line should provide a valuable tool for studying functions of germ cell-expressed genes involved in spermatogenesis. |
