| First Author | Xiong WH | Year | 2015 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 56 |
| Issue | 8 | Pages | 4961-74 |
| PubMed ID | 26230760 | Mgi Jnum | J:230947 |
| Mgi Id | MGI:5766578 | Doi | 10.1167/iovs.15-16622 |
| Citation | Xiong WH, et al. (2015) The Effect of PKCalpha on the Light Response of Rod Bipolar Cells in the Mouse Retina. Invest Ophthalmol Vis Sci 56(8):4961-74 |
| abstractText | PURPOSE: Protein kinase C alpha (PKCalpha) is abundantly expressed in rod bipolar cells (RBCs) in the retina, yet the physiological function of PKCalpha in these cells is not well understood. To elucidate the role of PKCalpha in visual processing in the eye, we examined the effect of genetic deletion of PKCalpha on the ERG and on RBC light responses in the mouse. METHODS: Immunofluorescent labeling was performed on wild-type (WT), TRPM1 knockout, and PKCalpha knockout (PKC-KO) retina. Scotopic and photopic ERGs were recorded from WT and PKC-KO mice. Light responses of RBCs were measured using whole-cell recordings in retinal slices from WT and PKC-KO mice. RESULTS: Protein kinase C alpha expression in RBCs is correlated with the activity state of the cell. Rod bipolar cells dendrites are a major site of PKCalpha phosphorylation. Electroretinogram recordings indicated that loss of PKCalpha affects the scotopic b-wave, including a larger peak amplitude, longer implicit time, and broader width of the b-wave. There were no differences in the ERG a- or c-wave between PKCalpha KO and WT mice, indicating no measurable effect of PKCalpha in photoreceptors or the RPE. The photopic ERG was unaffected consistent with the lack of detectable PKCalpha in cone bipolar cells. Whole-cell recordings from RBCs in PKC-KO retinal slices revealed that, compared with WT, RBC light responses in the PKC-KO retina are delayed and of longer duration. CONCLUSIONS: Protein kinase C alpha plays an important modulatory role in RBCs, regulating both the peak amplitude and temporal properties of the RBC light response in the rod visual pathway. |
