| Primary Identifier | MGI:3691483 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, RMCE-ready | Gene | Scn5a |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Recombinase-mediated cassette exchange was used to replace exon 2 with a loxP site, the full length human SCN5A, an FRT-flanked hygro cassette and a loxP site. Restriction fragment length polmorphisms were used to confirm that only the human transcripts were expressed in mutant hearts. This knock-in contains the conserved noncoding sequence 28 (CNS28). |
