| Primary Identifier | MGI:6718984 | Allele Type | Endonuclease-mediated |
| Attribute String | Reporter | Gene | Kras |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 genome editing is used to insert the Citrine cDNA with a linker sequence at the ATG start codon and to replace the site-directed mutagenized sequence for the oncogenic Kras protein in which the glycine at position 12 has been substituted to aspartic acid in the Kras locus so that the Citrine and oncogenic Kras G12D coding sequences are in the same reading frame. The construct replaced the LSLKrasG12D locus in frame with the KrasG12D gene in the male pronucleus of LSL-KrasG12D/+ mouse zygotes. |
