| First Author | Kita Y | Year | 1993 |
| Journal | J Immunol | Volume | 151 |
| Issue | 2 | Pages | 849-56 |
| PubMed ID | 8335914 | Mgi Jnum | J:13186 |
| Mgi Id | MGI:61393 | Doi | 10.4049/jimmunol.151.2.849 |
| Citation | Kita Y, et al. (1993) V gene analysis of anticardiolipin antibodies from MRL-lpr/lpr mice. J Immunol 151(2):849-56 |
| abstractText | We analyzed the VH and V kappa genes of 14 hybridomas producing anticardiolipin antibodies (aCL) from MRL-lpr/lpr mice, in an attempt to address the question of whether aCL are generated by Ag-driven stimulation or by polyclonal B cell activation. Five of six aCL from one mouse and both aCL from the other mouse carried 98 to 100% homologous VH and V kappa genes, respectively, thereby indicating that aCL were derived from the oligoclonal B cell precursor in that individual mouse. Of nine clones, six (67%) used the VH gene of the J558 family, and three (43%) of seven clones used the V kappa gene of the V kappa 23 group. Three to five somatic mutations were detected in all VH and V kappa genes of the examined aCL. These results suggest the possibility that usage of VH/V kappa genes in aCL is not random and that aCL consist of somatically mutated Ig genes. |
