| First Author | Bobé P | Year | 1993 |
| Journal | J Immunol | Volume | 151 |
| Issue | 5 | Pages | 2813-9 |
| PubMed ID | 7689616 | Mgi Jnum | J:14400 |
| Mgi Id | MGI:62569 | Doi | 10.4049/jimmunol.151.5.2813 |
| Citation | Bobe P, et al. (1993) Allogenic MHC class II determinant(s) in MRL/Ipr autoimmune disease-prone mice. Unusual expression of an L1 transposable element creates molecular mimicry. J Immunol 151(5):2813-9 |
| abstractText | In some aged MRL/Ipr autoimmune disease-prone mice, unexpected reactivity has been observed between lymphoid cells and mAb or polyclonal antibodies directed against the A beta d class II chain of the MHC. However, Southern blot analysis of their genomic DNA, using different class I and class II MHC-specific probes, confirmed their inbred character and their H-2k genotype. In this study, immunoprecipitation experiments with an anti-A beta d mAb indicated that the 45 (and 12)-kD molecule(s) recognized by an anti-A beta d mAb differed from a class II chain. After specific antibody screening of a lambda gt11 expression library constructed with MRL/Ipr A beta d-positive lymphoid cells, we cloned cDNA that share sequences with high homology (> 80%) to the 3' end of a 7-kb L1Md (L1) element propagated in the mouse genome via retrotransposition. Northern blot analysis showed an overtranscription of these L1 sequences in the MRL/Ipr spleen cells used for the construction of the cDNA library, in comparison to the MRL ancestor strains. Therefore, the autoimmune MRL/Ipr strain could express a translation product of L1 open reading frame 2, which mimics a highly antigenic epitope of an allogenic MHC class II Ag. |
