| First Author | Kanai Y | Year | 1993 |
| Journal | Biochem Biophys Res Commun | Volume | 196 |
| Issue | 2 | Pages | 729-36 |
| PubMed ID | 8240349 | Mgi Jnum | J:15414 |
| Mgi Id | MGI:63537 | Doi | 10.1006/bbrc.1993.2310 |
| Citation | Kanai Y, et al. (1993) Natural occurrence of Nuc in the sera of autoimmune-prone MRL/lpr mice. Biochem Biophys Res Commun 196(2):729-36 |
| abstractText | We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant(r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods. |
