| First Author | Ono M | Year | 1995 |
| Journal | Clin Exp Immunol | Volume | 100 |
| Issue | 2 | Pages | 284-90 |
| PubMed ID | 7743668 | Mgi Jnum | J:25558 |
| Mgi Id | MGI:73274 | Doi | 10.1111/j.1365-2249.1995.tb03666.x |
| Citation | Ono M, et al. (1995) Sequence analysis of the germ-line V-H gene corresponding to a nephritogenic antibody in MRL/lpr lupus mice. Clin Exp Immunol 100(2):284-290 |
| abstractText | In order to investigate the genetic origin of nephritogenic antibodies in MRL/Mp-lpr/lpr (MRL/lpr) lupus mice, we isolated the germ-line heavy chain variable region (V-H) gene corresponding to the nephritogenic antibody, B1, derived from an unmanipulated MRL/lpr mouse. Injection of this antibody into C.B-17/Icr-scid/scid mice resulted in the generation of wire loop-like glomerular lesions resembling those of lupus nephritis. Nucleotide sequences of this germ-line V-H gene showed no replacement mutation in the V-H region of the B1 antibody. Furthermore, this gene was identical to that found in the C3H/HeJ-lpr/lpr strain of mice. Our results suggest that germ-line V-H genes can encode nephritogenic antibodies without somatic mutation, even in a mouse strain not prone to lupus. |
