| First Author | Inaba T | Year | 2016 |
| Journal | Proc Natl Acad Sci U S A | Volume | 113 |
| Issue | 28 | Pages | 7834-9 |
| PubMed ID | 27342861 | Mgi Jnum | J:234331 |
| Mgi Id | MGI:5789812 | Doi | 10.1073/pnas.1603513113 |
| Citation | Inaba T, et al. (2016) Phospholipase Cbeta1 induces membrane tubulation and is involved in caveolae formation. Proc Natl Acad Sci U S A 113(28):7834-9 |
| abstractText | Lipid membrane curvature plays important roles in various physiological phenomena. Curvature-regulated dynamic membrane remodeling is achieved by the interaction between lipids and proteins. So far, several membrane sensing/sculpting proteins, such as Bin/amphiphysin/Rvs (BAR) proteins, are reported, but there remains the possibility of the existence of unidentified membrane-deforming proteins that have not been uncovered by sequence homology. To identify new lipid membrane deformation proteins, we applied liposome-based microscopic screening, using unbiased-darkfield microscopy. Using this method, we identified phospholipase Cbeta1 (PLCbeta1) as a new candidate. PLCbeta1 is well characterized as an enzyme catalyzing the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PIP2). In addition to lipase activity, our results indicate that PLCbeta1 possessed the ability of membrane tubulation. Lipase domains and inositol phospholipids binding the pleckstrin homology (PH) domain of PLCbeta1 were not involved, but the C-terminal sequence was responsible for this tubulation activity. Computational modeling revealed that the C terminus displays the structural homology to the BAR domains, which is well known as a membrane sensing/sculpting domain. Overexpression of PLCbeta1 caused plasma membrane tubulation, whereas knockdown of the protein reduced the number of caveolae and induced the evagination of caveolin-rich membrane domains. Taken together, our results suggest a new function of PLCbeta1: plasma membrane remodeling, and in particular, caveolae formation. |
