| First Author | McMichael AJ | Year | 1979 |
| Journal | Eur J Immunol | Volume | 9 |
| Issue | 3 | Pages | 205-10 |
| PubMed ID | 376318 | Mgi Jnum | J:17169 |
| Mgi Id | MGI:65221 | Doi | 10.1002/eji.1830090307 |
| Citation | McMichael AJ, et al. (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol 9(3):205-10 |
| abstractText | Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight. |
