| First Author | Falcone RA Jr | Year | 1999 |
| Journal | J Pediatr Surg | Volume | 34 |
| Issue | 5 | Pages | 800-4 |
| PubMed ID | 10359184 | Mgi Jnum | J:57179 |
| Mgi Id | MGI:1344060 | Doi | 10.1016/s0022-3468(99)90376-7 |
| Citation | Falcone RA Jr, et al. (1999) The adaptive intestinal response to massive enterectomy is preserved in c-SRC-deficient mice. J Pediatr Surg 34(5):800-4 |
| abstractText | BACKGROUND/PURPOSE: The Src family of protein tyrosine kinases has been implicated in the downstream mitogenic signaling of several ligands including epidermal growth factor (EGF). Because EGF likely plays a role in adaptation after massive small bowel resection (SBR), we tested the hypothesis that c-src is required for this important response. METHODS: A 50% proximal SBR or sham operation (bowel transection or reanastomosis alone) was performed on c-src-deficient (n = 14) or wild-type (C57bl/6) mice (n = 20). The ileum was harvested on postoperative day 3 and adaptive parameters determined as changes in ileal wet weight, protein and DNA content, proliferation index, villus height, and crypt depth. Comparisons were done using analysis of variance (ANOVA), and a Pvalue less than .05 was considered significant. Values are presented as mean +/- SEM. RESULTS: The activity of c-src was increased in the ileum of wild-type mice after SBR but remained unchanged in c-src-deficient mice. Despite this lack of increase, adaptation occurred after SBR in the c-src-deficient mice as demonstrated by increased ileal wet weight, protein and DNA content, proliferation index, villus height, and crypt depth similar to wild-type mice. CONCLUSIONS: The adaptive response of the intestine to massive SBR is preserved despite reduced activity of the c-src protein. The mitogenic signaling that characterizes intestinal adaptation and is associated with receptor activation by EGF or other growth factors probably occurs by mechanisms independent of c-src protein tyrosine kinase. |
