| First Author | Meyuhas O | Year | 1990 |
| Journal | J Biol Chem | Volume | 265 |
| Issue | 20 | Pages | 11465-73 |
| PubMed ID | 2365680 | Mgi Jnum | J:10605 |
| Mgi Id | MGI:59053 | Doi | 10.1016/s0021-9258(19)38420-0 |
| Citation | Meyuhas O, et al. (1990) The mouse ribosomal protein L7 gene. Its primary structure and functional analysis of the promoter region. J Biol Chem 265(20):11465-73 |
| abstractText | The expressed gene coding for mouse ribosomal protein L7 (rpL7) was structurally and functionally characterized. It consists of seven exons, spans 3107 base pairs, and its coding sequence initiates within exon 1. The primary structure of mouse rpL7 (270 amino acids), as inferred from the nucleotide sequence of the exons of the gene, and from the cDNA, is 12 residues longer than the rat counterpart. The rpL7 gene shares common structural features with most other mammalian ribosomal protein genes analyzed thus far. These include the lack of a canonical TATA box and a major transcription initiation site at a cytidine residue embedded in a stretch of 14 pyrimidines, flanked by C + G-rich regions. Transient expression assays revealed that the promoter region of rpL7 gene bears several regulatory elements, both upstream to the capsite and within the transcribed portion of the gene. One internal regulatory element was assigned to the first intron and a second one to a 20-base pair region spanning the first exon-intron junction. The activity of a deletion mutant of rpL32 gene, lacking its internal elements can be rescued by insertion, in the sense orientation, of the corresponding elements from the rpL7 gene. The unique spatial organization of the regulatory elements in rpL7 gene, as well as in other murine ribosomal protein genes examined thus far, might indicate that this common architecture is involved in the mechanism coordinating their expression. |
