| First Author | Marquez B | Year | 2008 |
| Journal | Reprod Fertil Dev | Volume | 20 |
| Issue | 2 | Pages | 247-52 |
| PubMed ID | 18255013 | Mgi Jnum | J:139823 |
| Mgi Id | MGI:3810211 | Doi | 10.1071/rd07146 |
| Citation | Marquez B, et al. (2008) Soluble adenylyl cyclase is required for activation of sperm but does not have a direct effect on hyperactivation. Reprod Fertil Dev 20(2):247-52 |
| abstractText | Soluble adenylyl cyclase (SACY) is an essential component of cAMP-signalling cascades that activate sperm motility and capacitate sperm. SACY activity is stimulated by HCO(3)(-) and Ca(2+). Sperm from Sacy(-/-) (null) mice were immotile or weakly motile, but cAMP analogues N(6),2'-O-dibutyryladenosine 3',5'-cyclic monophosphate (dbcAMP) and adenosine 3',5'-cyclic monophosphate acetoxymethyl ester (cAMP-AM) activated motility. Null sperm activated by dbcAMP quickly developed hairpin bends at the junction of the midpiece and principal piece, which could be prevented by omitting HCO(3)(-). Treating Sacy(-/-) sperm with thimerosal or NH(4)Cl to raise flagellar cytoplasmic Ca(2+) could not substitute for cAMP analogues in activating motility; however, sperm activated with cAMP-AM hyperactivated after thimerosal treatment. Treating activated wild-type sperm with SACY inhibitor KH7 did not prevent hyperactivation from developing during capacitation in vitro, although high doses impaired motility. These results indicate that, while the SACY/cAMP signalling pathway is required for motility activation, it is not directly involved in triggering hyperactivation. |
