| Primary Identifier | MGI:6473983 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Dnah8 |
| Strain of Origin | (C57BL/6 x DBA/2)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology used gRNAs (gRNA #1: 5'-GCCACCCCCTCCGAGTGAAG-3' and gRNA #2: 5'-TAGAATCAGAAGCGTGAATT-3') targeting exons 2 and 93 to delete the entire coding region which resulted in a 239,922 bp deletion plus a 4 bp insertion. Western blot analysis confirmed the absence of protein in the testis of homozygous mice. |
