| Primary Identifier | MGI:7444782 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Reporter | Gene | Tg(CAG-Katshuka)13Sgo |
| Strain of Origin | (C57BL/6JOlaHsd x CBA/J)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgenic construct consists of the ubiquitous promoter CAG, composed of the cytomegalovirus (CMV) immediate early enhancer and the modified chicken b-actin promoter, a loxP-flanked transcription STOP cassette (LSL) followed by the cDNA of Katushka fluorescent protein (KFP), and a rabbit beta-globin polyadenylation signal. The Katushka expression is driven by the CAG promoter upon Cre recombinase-mediated excision of the floxed STOP cassette. Five founder mice were generated, three of which transmitted germline. Two lines 3 and 13 which contained a single integration event, were further expanded and characterized. No significant differences were observed between them. Most of the studies were performed with line 3. |
