First Author | Nangaku M | Year | 1994 |
Journal | Cell | Volume | 79 |
Issue | 7 | Pages | 1209-20 |
PubMed ID | 7528108 | Mgi Jnum | J:22228 |
Mgi Id | MGI:70109 | Doi | 10.1016/0092-8674(94)90012-4 |
Citation | Nangaku M, et al. (1994) KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria. Cell 79(7):1209-20 |
abstractText | To further elucidate the mechanism of organelle transport, we cloned a novel member of the mouse kinesin superfamily, KIF1B. This N-terminal-type motor protein is expressed ubiquitously in various kinds of tissues. In situ hybridization revealed that KIF1B is expressed abundantly in differentiated nerve cells. Interestingly, K1F1B works as a monomer, having a microtubule plus end-directed motility. Our rotary shadowing electron microscopy revealed mostly single globular structures. Immunocytochemically, KIF1B was colocalized with mitochondria in vivo. Furthermore, a subcellular fractionation study showed that KIF1B was concentrated in the mitochondrial fraction, and purified K1F1B could transport mitochondria along microtubules in vitro. These data strongly suggested that KIF1B works as a monomeric motor for anterograde transport of mitochondria. |