| First Author | Friocourt G | Year | 2001 |
| Journal | Mol Cell Neurosci | Volume | 18 |
| Issue | 3 | Pages | 307-19 |
| PubMed ID | 11591131 | Mgi Jnum | J:72428 |
| Mgi Id | MGI:2152662 | Doi | 10.1006/mcne.2001.1022 |
| Citation | Friocourt G, et al. (2001) Doublecortin interacts with mu; subunits of clathrin adaptor complexes in the developing nervous system. Mol Cell Neurosci 18(3):307-19 |
| abstractText | Doublecortin is a microtubule-associated protein required for normal corticogenesis in the developing brain. We carried out a yeast two-hybrid screen to identify interacting proteins. One of the isolated clones encodes the &mgr;1 subunit of the adaptor complex AP-1 involved in clathrin-dependent protein sorting. We found that Doublecortin also interacts in yeast with &mgr;2 from the AP-2 complex. Mutagenesis and pull-down experiments showed that these interactions were mediated through a tyrosine-based sorting signal (YLPL) in the C-terminal part of Doublecortin. The functional relevance of these interactions was suggested by the coimmunoprecipitation of Doublecortin with AP-1 and AP-2 from mouse brain extracts. This interaction was further supported by RNA in situ hybridization and immunofluorescence studies. Taken together these data indicate that a certain proportion of Doublecortin interacts with AP-1 and/or AP-2 in vivo and are consistent with a potential involvement of Doublecortin in protein sorting or vesicular trafficking. |
