| Primary Identifier | MGI:2155485 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Smad1 |
| Inheritance Mode | Recessive | Transmission | Germline |
| Strain of Origin | 129S/SvEv-Gpi1<c> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | This allele was generated by crossing mice carrying the Smad1tm2Rob allele to mice carrying a Cre transgene controlled by a mouse protamine 1 promoter that directs Cre-recombinase expression specifically to the male germline. An in vivo Cre-mediated excision removed all of the first coding exon and approximately 2kb of 5' and 3' flanking genomic sequences, and left a single loxP and EcoRI site in the locus. Northern analysis of 9.5dpc homozygous embryos using a probe for the 3' UTR detected a shorter transcript, but not the wild-type transcript. Western blot analysis using an antibody that recognizes the MH2 domain did not detect intact or truncated protein in 9.5dpc homozygous embryos. |
