| Primary Identifier | MGI:5770554 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Prm2 |
| Strain of Origin | (C57BL/6 x DBA)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9-mediated genome editing generated a 101bp deletion in exon 1 leading to a frameshift that results in nonsense transcripts with a premature stop codon after amino acid 23. Western blot analysis of basic nuclear proteins isolated from epididymal sperm confirmed the absence of detectable mature protein in homozygous mutant males. |
