| First Author | Chaprov KD | Year | 2020 |
| Journal | Dokl Biol Sci | Volume | 494 |
| Issue | 1 | Pages | 260-263 |
| PubMed ID | 33083886 | Mgi Jnum | J:327455 |
| Mgi Id | MGI:6884620 | Doi | 10.1134/S0012496620050014 |
| Citation | Chaprov KD, et al. (2020) Increased Expression of the Multimerin-1 Gene in alpha-Synuclein Knokout Mice. Dokl Biol Sci 494(1):260-263 |
| abstractText | Multimerin-1 (Mmrn-1) is a soluble protein, also known as elastin microfibril interfacer 4 (EMILIN-4), found in platelets and in the endothelium of blood vessels. Its function and role in pathology are still not fully understood. Genetic modifications in alpha-synuclein gene (Snca) locus that mapped 160 Kb apart from Mmrn-1 in mouse genome, could weigh with regulatory elements of Mmrn-1 gene. We have studied the Mmrn-1 expression in brain cortex of three mouse lines with Snca knock-out: B6(Cg)-Snca(tm1.2Vlb)/J, B6;129-Snca(tm1Sud)/J, and B6;129X1-Snca(tm1Rosl)/J. The 35-fold increase for Mmrn-1 mRNA level have been found in B6;129X1-Snca(tm1Rosl)/J mice that carry in their genome foreign sequences including bacterial gene neo and a strong promoter of a mouse phosphoglycerate kinase (Pgk1) oriented towards Mmrn-1 gene. This effect on regulatory elements of Mmrn-1 gene as a result of modifications in Snca locus should be taken into consideration when using B6;129X1-Snca(tm1Rosl)/J line, that is widely applied for study of neurodegeneration mechanisms. |
