| First Author | Zhang Y | Year | 2023 |
| Journal | iScience | Volume | 26 |
| Issue | 8 | Pages | 107464 |
| PubMed ID | 37588169 | Mgi Jnum | J:340872 |
| Mgi Id | MGI:7519867 | Doi | 10.1016/j.isci.2023.107464 |
| Citation | Zhang Y, et al. (2023) IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4(+)T cells. iScience 26(8):107464 |
| abstractText | Vaccination is an effective means of preventing pneumococcal disease and SPY1 is a live attenuated pneumococcal vaccine we obtained earlier. We found IL-27 and its specific receptor (WSX-1) were increased in SPY1 vaccinated mice. Bacterial clearance and survival rates were decreased in SPY1 vaccinated IL-27Ralpha(-/-) mice. The vaccine-induced Th17 cell response and IgA secretion were also suppressed in IL-27Ralpha(-/-) mice. STAT3 and NF-kappaB signaling and expression of the Th17 cell polarization-related cytokines were also decreased in IL-27Ralpha(-/-) bone-marrow-derived dendritic cells(BMDC) stimulated with inactivated SPY1. The numbers of memory CD4(+)T cells were also decreased in SPY1 vaccinated IL-27Ralpha(-/-) mice. These results suggested that IL-27 plays a protective role in SPY1 vaccine by promoting Th17 polarization through STAT3 and NF-kappaB signaling pathways and memory CD4(+)T cells production in the SPY1 vaccine. In addition, we found that the immune protection of SPY1 vaccine was independent of aerobic glycolysis. |
