| First Author | Sharma A | Year | 2022 |
| Journal | J Immunol | Volume | 208 |
| Issue | 8 | Pages | 2008-2018 |
| PubMed ID | 35354611 | Mgi Jnum | J:325673 |
| Mgi Id | MGI:7281042 | Doi | 10.4049/jimmunol.2000945 |
| Citation | Sharma A, et al. (2022) IL-27 Enhances gammadelta T Cell-Mediated Innate Resistance to Primary Hookworm Infection in the Lungs. J Immunol 208(8):2008-2018 |
| abstractText | IL-27 is a heterodimeric IL-12 family cytokine formed by noncovalent association of the promiscuous EBI3 subunit and selective p28 subunit. IL-27 is produced by mononuclear phagocytes and unfolds pleiotropic immune-modulatory functions through ligation to IL-27 receptor alpha (IL-27RA). Although IL-27 is known to contribute to immunity and to limit inflammation after various infections, its relevance for host defense against multicellular parasites is still poorly defined. Here, we investigated the role of IL-27 during infection with the soil-transmitted hookworm, Nippostrongylus brasiliensis, in its early host intrapulmonary life cycle. IL-27(p28) was detectable in bronchoalveolar lavage fluid of C57BL/6J wild-type mice on day 1 after s.c. inoculation. IL-27RA expression was most abundant on lung-invading gammadelta T cells. Il27ra(-/-) mice showed increased lung parasite burden together with aggravated pulmonary hemorrhage and higher alveolar total protein leakage as a surrogate for epithelial-vascular barrier disruption. Conversely, injections of recombinant mouse (rm)IL-27 into wild-type mice reduced lung injury and parasite burden. In multiplex screens, higher airway accumulations of IL-6, TNF-alpha, and MCP-3 (CCL7) were observed in Il27ra(-/-) mice, whereas rmIL-27 treatment showed a reciprocal effect. Importantly, gammadelta T cell numbers in airways were enhanced by endogenous or administered IL-27. Further analysis revealed a direct antihelminthic function of IL-27 on gammadelta T cells as adoptive intratracheal transfer of rmIL-27-treated gammadelta T cells during primary N. brasiliensis lung infection conferred protection in mice. In summary, this report demonstrates protective functions of IL-27 to control the early lung larval stage of hookworm infection. |
