| First Author | Beiersdorfer A | Year | 2019 |
| Journal | Glia | Volume | 67 |
| Issue | 7 | Pages | 1385-1400 |
| PubMed ID | 30883940 | Mgi Jnum | J:275238 |
| Mgi Id | MGI:6304801 | Doi | 10.1002/glia.23613 |
| Citation | Beiersdorfer A, et al. (2019) Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca(2+) transients and neurovascular coupling. Glia 67(7):1385-1400 |
| abstractText | Astrocytes are arranged in highly organized gap junction-coupled networks, communicating via the propagation of Ca(2+) waves. Astrocytes are gap junction-coupled not only to neighboring astrocytes, but also to oligodendrocytes, forming so-called panglial syncytia. It is not known, however, whether glial cells in panglial syncytia transmit information using Ca(2+) signaling. We used confocal Ca(2+) imaging to study intercellular communication between astrocytes and olfactory ensheathing glial cells (OECs) in in-toto preparations of the mouse olfactory bulb. Our results demonstrate that Ca(2+) transients in juxtaglomerular astrocytes, evoked by local photolysis of "caged" ATP and "caged" tACPD, led to subsequent Ca(2+) responses in OECs. This transmission of Ca(2+) responses from astrocytes to OECs persisted in the presence of neuronal inhibition, but was absent when gap junctional coupling was suppressed with carbenoxolone. When Ca(2+) transients were directly evoked in OECs by puff application of DHPG, they resulted in delayed Ca(2+) responses in juxtaglomerular astrocytes, indicating that panglial transmission of Ca(2+) signals occurred in a bidirectional manner. In addition, panglial transmission of Ca(2+) signals from astrocytes to OECs resulted in vasoconstriction of OEC-associated blood vessels in the olfactory nerve layer. Our results demonstrate functional transmission of Ca(2+) signals between different classes of glial cells within gap junction-coupled panglial networks and the resulting regulation of blood vessel diameter in the olfactory bulb. |
