| First Author | Xie J | Year | 2009 |
| Journal | J Cutan Pathol | Volume | 36 |
| Issue | 11 | Pages | 1146-50 |
| PubMed ID | 19469869 | Mgi Jnum | J:154722 |
| Mgi Id | MGI:4397773 | Doi | 10.1111/j.1600-0560.2009.01246.x |
| Citation | Xie J, et al. (2009) Effects of antisense oligodeoxynucleotide to type I collagen gene on hypertrophic scars in the transplanted nude mouse model. J Cutan Pathol 36(11):1146-50 |
| abstractText | BACKGROUND: Antisense nucleic acids are effective in inhibiting harmful or uncontrolled gene expression. We had previously proved that the antisense DNA to type I collagen could effectively inhibit the synthesis of collagen type I in cultured hypertrophic scar fibroblasts, suggesting a potential role in anti-scarring, but there are no published reports of its effect on scar in the transplanted nude mouse model. AIMS: To investigate the effects of antisense oligodeoxynucleotide (ASODN) to type I collagen gene on hypertrophic scars in the transplanted nude mouse model and clarify the potential of ASODN for the treatment of scars. METHODS: The nudemousemodel of hypertrophic scar was created and subjected to daily injections with ASODN and LipofectamineTM for 2 ,4 or 6 weeks.We then examined the scars for changes in histopathological characteristics. The effects of ASODNon type I collagen gene expression were examined by reverse transcription-polymerase chain reaction and Western blots. RESULTS: The ASODN could remarkably alleviate the scar in the nude mouse model and consistently inhibit type I collagen gene expression at both the mRNA and protein levels. CONCLUSION: ASODN was effective in downregulating type I collagen gene expression and could prove to be useful in the treatment of scars. |
