| Primary Identifier | MGI:6116833 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Iho1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Clone HTGRS6006_A_G10 from the BACPAC Resources Center, Knockout Mouse Project KOMP repository was used to target embryonic stem cells. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. The first loxP site is followed by a neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3. Exon 3 is thus flanked by loxP sites. Western blot analysis confirmed absence of the full-length protein in testis. |
