| First Author | Clift D | Year | 2017 |
| Journal | Cell | Volume | 171 |
| Issue | 7 | Pages | 1692-1706.e18 |
| PubMed ID | 29153837 | Mgi Jnum | J:255584 |
| Mgi Id | MGI:6110532 | Doi | 10.1016/j.cell.2017.10.033 |
| Citation | Clift D, et al. (2017) A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171(7):1692-1706.e18 |
| abstractText | Methods for the targeted disruption of protein function have revolutionized science and greatly expedited the systematic characterization of genes. Two main approaches are currently used to disrupt protein function: DNA knockout and RNA interference, which act at the genome and mRNA level, respectively. A method that directly alters endogenous protein levels is currently not available. Here, we present Trim-Away, a technique to degrade endogenous proteins acutely in mammalian cells without prior modification of the genome or mRNA. Trim-Away harnesses the cellular protein degradation machinery to remove unmodified native proteins within minutes of application. This rapidity minimizes the risk that phenotypes are compensated and that secondary, non-specific defects accumulate over time. Because Trim-Away utilizes antibodies, it can be applied to a wide range of target proteins using off-the-shelf reagents. Trim-Away allows the study of protein function in diverse cell types, including non-dividing primary cells where genome- and RNA-targeting methods are limited. |
