| First Author | Kaufmann Y | Year | 1991 |
| Journal | J Immunol | Volume | 147 |
| Issue | 1 | Pages | 369-74 |
| PubMed ID | 2051027 | Mgi Jnum | J:11253 |
| Mgi Id | MGI:59692 | Doi | 10.4049/jimmunol.147.1.369 |
| Citation | Kaufmann Y, et al. (1991) Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells. J Immunol 147(1):369-74 |
| abstractText | The lymphocyte function-associated molecule 1 (LFA-1, CD11a/CD18) is an integrin that mediates adhesion of immune cells by interaction with two members of the Ig superfamily, ICAM-1 and ICAM-2. LFA-1 consists of an alpha subunit (Mr = 180,000) and a beta subunit (Mr = 95,000). We report here the isolation and expression of the murine alpha subunit cDNA (GenBank accession no. M60778). The deduced sequence comprises a 1061 amino acid extracellular domain, a 29 amino acid transmembrane region, and a 50 amino acid cytoplasmic domain. It has a 72% amino acid identity with its human counterpart and 34% identity with the murine Mac-1 alpha subunit. The murine LFA-1 alpha subunit could be expressed on the cell surface of a fibroblastoid cell line, COS, by cotransfection with either the human or murine beta subunit cDNA. |
