| First Author | Ahras M | Year | 2008 |
| Journal | J Lipid Res | Volume | 49 |
| Issue | 7 | Pages | 1569-76 |
| PubMed ID | 18375996 | Mgi Jnum | J:138460 |
| Mgi Id | MGI:3805182 | Doi | 10.1194/jlr.M800055-JLR200 |
| Citation | Ahras M, et al. (2008) Scavenger receptor class B type I localizes to a late endosomal compartment. J Lipid Res 49(7):1569-76 |
| abstractText | Scavenger receptor class B type I (SR-BI) has an established role in mediating the selective uptake of cholesterol from HDL in hepatocytes, steroidogenic cells, and other tissues. SR-BI is present on the plasma membrane but also localizes to stable intracellular compartments of unknown function. Using indirect immunofluorescence and subcellular fractionation, we have investigated the subcellular distribution of SR-BI. We report that red fluorescent protein-tagged mouse SR-BI (RFP-mSR-BI) colocalizes with the late endosomal and lysosomal markers, Rab7, LBPA, and Rab9. In addition, endogenous SR-BI is also found on lysosomes and colocalizes with LAMP-2 in primary hepatocytes. Furthermore, we demonstrate that the trafficking of SR-BI through these compartments is Rab7 dependent. Interestingly, filipin staining indicates accumulation of lysosomal cholesterol in SR-BI-deficient ((-/-)) as compared with wild-type hepatocytes. In addition to its role as a plasma membrane receptor, SR-BI may function in cholesterol trafficking from late endosomes/lysosomes. |
