| First Author | Pogulis RJ | Year | 1995 |
| Journal | Gene | Volume | 158 |
| Issue | 2 | Pages | 231-5 |
| PubMed ID | 7607547 | Mgi Jnum | J:36031 |
| Mgi Id | MGI:83473 | Doi | 10.1016/0378-1119(95)00157-2 |
| Citation | Pogulis RJ, et al. (1995) Murine collagen intron-binding factor I (CIBF-I) is the same protein as transcription factor Oct-1. Gene 158(2):231-5 |
| abstractText | The recognition sequence (CIB) of collagen intron-binding factor I (CIBF-I) loosely resembles the consensus octamer-binding motif (OCT). In the present study we investigate whether CIBF-I is actually the OCT-binding protein, Oct-1. Electrophoretic mobility-shift assays (EMSA) demonstrate that a consensus OCT motif effectively competes for CIBF-I binding. CIBF-I and Oct-1 complexes display similar EMSA characteristics, and both factors are detected in nuclear extracts of five different cell types. In addition, pre-incubation of nuclear extracts with antiserum directed against the POU domain of Oct-1 inhibits CIBF-I complex formation. Finally, DNA transfection experiments demonstrate that a single copy of the CIB site is sufficient to stimulate transcription from the SV40 early promoter in NIH 3T3 cells. These results suggest that CIBF-I is the ubiquitously distributed OCT-binding protein, Oct-1, and represent the first report that an octamer-binding protein contributes to the transcriptional activity of a collagen-encoding gene. |
