| Primary Identifier | MGI:6356540 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Xpo1 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed to insert a cassette containing an inverted exons 15-17 with a mutated exon 16 downstream of endogenous exon 17 of the gene. Specifically, the vector includes, from 5' to 3', a loxP site, endogenous exons 15-17, an FRT-flanked neomycin resistance (neo) cassette, a lox71 site, a cassette containing inverted exons 15-17, a lox66 site. The inverted cassette is modified to harbor a guanine to adenine substitution at amino acid 571 in exon 16, resulting in a glutamic acid to lysine mutation (E571K), commonly found in patients with a variety of cancer types, including chronic lymphocytic leukemia (CLL), Hodgkin's lymphoma (HL), and esophageal carcinoma. Flp-mediated recombination removed the FRT-flanked neo cassette. |
