| First Author | Hagios C | Year | 1999 |
| Journal | Exp Cell Res | Volume | 253 |
| Issue | 2 | Pages | 607-17 |
| PubMed ID | 10585284 | Mgi Jnum | J:58940 |
| Mgi Id | MGI:1350703 | Doi | 10.1006/excr.1999.4658 |
| Citation | Hagios C, et al. (1999) Tenascin-Y, a component of distinctive connective tissues, supports muscle cell growth. Exp Cell Res 253(2):607-17 |
| abstractText | Chicken tenascin-Y is an extracellular matrix protein most closely related to the mammalian tenascin-X. It is highly expressed in the connective tissue of skeletal muscle (C. Hagios, M. Koch, J. Spring, M. Chiquet, and R. Chiquet-Ehrismann, 1996, J. Cell Biol. 134, 1499-1512). Here we demonstrate the presence of tenascin-Y in specific areas of the connective tissues in developing lung, kidney, and skin. In skin tenascin-Y shows a complementary expression pattern to tenascin-C, whereas in the lung and kidney the sites of expression are partly overlapping. Tenascin-Y is also present in embryonic skeletal muscle where it is expressed in the developing connective tissue in between the muscle fibers. This connective tissue is also the major site of alpha5 integrin expression. We purified recombinantly expressed tenascin-Y and tested its effect on cell adhesion and its influence on muscle cell growth and differentiation. C2C12 myoblasts were able to adhere to tenascin-Y and showed extensive formation of actin-rich processes without generation of stress fibers. Furthermore, we found that tenascin-Y influenced cell morphology of chick embryo fibroblasts over prolonged times in culture and that it supports primary muscle cell growth and restricts muscle cell differentiation. Copyright 1999 Academic Press. |
