| First Author | Kinnebrew MA | Year | 2012 |
| Journal | Immunity | Volume | 36 |
| Issue | 2 | Pages | 276-87 |
| PubMed ID | 22306017 | Mgi Jnum | J:181332 |
| Mgi Id | MGI:5311056 | Doi | 10.1016/j.immuni.2011.12.011 |
| Citation | Kinnebrew MA, et al. (2012) Interleukin 23 Production by Intestinal CD103(+)CD11b(+) Dendritic Cells in Response to Bacterial Flagellin Enhances Mucosal Innate Immune Defense. Immunity 36(2):276-87 |
| abstractText | Microbial penetration of the intestinal epithelial barrier triggers inflammatory responses that include induction of the bactericidal C-type lectin RegIIIgamma. Systemic administration of flagellin, a bacterial protein that stimulates Toll-like receptor 5 (TLR5), induces epithelial expression of RegIIIgamma and protects mice from intestinal colonization with antibiotic-resistant bacteria. Flagellin-induced RegIIIgamma expression is IL-22 dependent, but how TLR signaling leads to IL-22 expression is incompletely defined. By using conditional depletion of lamina propria dendritic cell (LPDC) subsets, we demonstrated that CD103(+)CD11b(+) LPDCs, but not monocyte-derived CD103(-)CD11b(+) LPDCs, expressed high amounts of IL-23 after bacterial flagellin administration and drove IL-22-dependent RegIIIgamma production. Maximal expression of IL-23 subunits IL-23p19 and IL-12p40 occurred within 60 min of exposure to flagellin. IL-23 subsequently induced a burst of IL-22 followed by sustained RegIIIgamma expression. Thus, CD103(+)CD11b(+) LPDCs, in addition to promoting long-term tolerance to ingested antigens, also rapidly produce IL-23 in response to detection of flagellin in the lamina propria. |
