| First Author | Kim HJ | Year | 2022 |
| Journal | Redox Biol | Volume | 54 |
| Pages | 102386 | PubMed ID | 35809434 |
| Mgi Jnum | J:346553 | Mgi Id | MGI:7316010 |
| Doi | 10.1016/j.redox.2022.102386 | Citation | Kim HJ, et al. (2022) Vitamin A aldehyde-taurine adducts function in photoreceptor cells. Redox Biol 54:102386 |
| abstractText | To facilitate the movement of retinoids through the visual cycle and to limit nonspecific chemical reaction, multiple mechanisms are utilized to handle these molecules when not contained within the binding pocket of opsin. Vitamin A aldehyde is sequestered by reversible Schiff base formation with phosphatidylethanolamine (PE) and subsequently undergoes NADPH-dependent reduction. Otherwise inefficient handling of retinaldehyde can lead to the formation of fluorescent di-retinal compounds within the outer segments of photoreceptor cells. These bisretinoid fluorophores initiate photooxidative processes having adverse consequences for retina. Various carrier proteins confer water solubility and maintain the 11-cis-retinoid configuration. Mechanisms for sequestration of retinoid include the formation of a reversible Schiff base between retinaldehyde and taurine (A1-taurine, A1T), the most abundant amino acid in photoreceptor cells. Here we have undertaken to examine the effects of taurine depletion using the transport inhibitors guanidinoethyl sulfonate (GES) and beta-alanine. Oral treatment of BALB/cJ mice with beta-alanine reduced ocular A1T and the mice exhibited significantly lower scotopic and photopic a-wave amplitudes. As a secondary effect of retinal degeneration, A1T was not detected and taurine was significantly reduced in mice carrying a P23H opsin mutation. The thinning of ONL that is indicative of reduced photoreceptor cell viability in albino Abca4(-/-) mice was more pronounced in beta-alanine treated mice. Treatment of agouti and albino Abca4(-/-) mice with beta-alanine and GES was associated with reduced bisretinoid measured chromatographically. Consistent with a reduction in carbonyl scavenging activity by taurine, methylglyoxal-adducts were also increased in the presence of beta-alanine. Taken together these findings support the postulate that A1T serves as a reservoir of vitamin A aldehyde, with diminished A1T explaining reduced photoreceptor light-sensitivity, accentuated ONL thinning in Abca4(-/-) mice and attenuated bisretinoid formation. |
