| Primary Identifier | MGI:4835664 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Runx1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cellular IRES in the 5' UTR of exon 3, the coding region of exon 3 and part of intron 3 were replaced with a floxed neo cassette and a Runx1 and Mecom fusion cDNA via homologous recombination. The neo cassette was removed by cre mediated recombination. RT_PCR confirmed the expression of the fusion gene from both endogenous promoters. Western blot analysis confirmed expression of the fusion protein. |
