| First Author | Thakore P | Year | 2021 |
| Journal | Elife | Volume | 10 |
| PubMed ID | 33635784 | Mgi Jnum | J:316946 |
| Mgi Id | MGI:6727738 | Doi | 10.7554/eLife.63040 |
| Citation | Thakore P, et al. (2021) Brain endothelial cell TRPA1 channels initiate neurovascular coupling. Elife 10:e63040 |
| abstractText | Cerebral blood flow is dynamically regulated by neurovascular coupling to meet the dynamic metabolic demands of the brain. We hypothesized that TRPA1 channels in capillary endothelial cells are stimulated by neuronal activity and instigate a propagating retrograde signal that dilates upstream parenchymal arterioles to initiate functional hyperemia. We find that activation of TRPA1 in capillary beds and post-arteriole transitional segments with mural cell coverage initiates retrograde signals that dilate upstream arterioles. These signals exhibit a unique mode of biphasic propagation. Slow, short-range intercellular Ca(2+) signals in the capillary network are converted to rapid electrical signals in transitional segments that propagate to and dilate upstream arterioles. We further demonstrate that TRPA1 is necessary for functional hyperemia and neurovascular coupling within the somatosensory cortex of mice in vivo. These data establish endothelial cell TRPA1 channels as neuronal activity sensors that initiate microvascular vasodilatory responses to redirect blood to regions of metabolic demand. |
