| First Author | Shin J | Year | 2023 |
| Journal | Am J Physiol Cell Physiol | Volume | 325 |
| Issue | 2 | Pages | C509-C518 |
| PubMed ID | 37486067 | Mgi Jnum | J:341255 |
| Mgi Id | MGI:7515055 | Doi | 10.1152/ajpcell.00157.2023 |
| Citation | Shin J, et al. (2023) MicroRNA-140 is not involved in sepsis-induced muscle atrophy. Am J Physiol Cell Physiol 325(2):C509-C518 |
| abstractText | Sepsis is a life-threatening inflammatory response to infection, often accompanied by skeletal muscle atrophy. A previous study demonstrated that the administration of microRNA-140 (miR-140) attenuated lipopolysaccharide (LPS)-induced muscle atrophy, whereas miR-140 knockdown with siRNA promoted atrophy. Therefore, we investigated whether miR-140 is involved in LPS-induced muscle atrophy using a genetic model, miR-140(-/-) mice. We found that a single injection of LPS induced atrophy both in slow-twitch and fast-twitch muscles. The muscle weights and fiber cross-sectional areas were significantly reduced in both the wild-type (WT) and miR-140(-/-) mice, with no difference between genotypes. The expression of several proteolysis markers, muscle-specific RING-finger 1 (MuRF1) and MAFbx/atrogin-1, increased in both groups after LPS injection. The ubiquitinated proteins in the miR-140(-/-) mice were similar to those in the WT mice. Therefore, the deletion of miR-140 did not affect LPS-induced muscle atrophy.NEW & NOTEWORTHY We used miR-140(-/-) mice to determine the function of miR-140 in LPS-induced skeletal muscle atrophy. To our knowledge, this study is the first to examine slow-twitch muscles in LPS-induced muscle wasting after miR-140 manipulation. |
