| Primary Identifier | MGI:6437907 | Allele Type | Endonuclease-mediated |
| Attribute String | Reporter | Gene | Abcb1a |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 genome editing is used to replace the stop codon with a bicistronic reporter containing a P2A peptide sequence and the fluorescent reporter ametrine. In addition, an in-frame 9 nucleotide deletion is found near the 3' end of the gene. The deletion is not believed to affect protein expression. Ametrine is a long stokes shift (excitation at 406 nanometers and emission at 526 nanometers) yellow fluorescent protein derived from Aequorea victoria. |
