| First Author | Quinn G | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 276 |
| Issue | 3 | Pages | 1089-99 |
| PubMed ID | 11027595 | Mgi Jnum | J:65110 |
| Mgi Id | MGI:1891787 | Doi | 10.1006/bbrc.2000.3602 |
| Citation | Quinn G, et al. (2000) Mouse flt-1 promoter directs endothelial-specific expression in the embyroid body model of embryogenesis. Biochem Biophys Res Commun 276(3):1089-99 |
| abstractText | The endothelial-specific receptor tyrosine kinase flt-1 (VEGFR-1) is expressed early on during endothelial lineage commitment both in vivo and in vitro. However, the exact function of flt-1 in vascular development still remains unclear. Here we report that a 2.2-kb fragment 5' of the mouse flt-1 gene becomes transcriptionally active during endothelial cell differentiation in developing embryoid bodies derived from mouse ES cells. Reporter gene expression correlated well with PECAM-1 expression and mirrored the expression pattern of the endogenous flt-1 gene. The temporal and spatial activity of the 2.2-kb flt-1 promoter provides a means to (1) identify a living population of early committed endothelial/bipotential progenitors and (2) ectopically express biologically active genes during lineage commitment. Copyright 2000 Academic Press. |
